A versatile toolbox for knock-in gene targeting based on the Multisite Gateway technology.
Yoshimatsu S, Sone T, Nakajima M, Sato T, Okochi R, Ishikawa M, Nakamura M, Sasaki E, Shiozawa S, Okano H
PLoS One. 2019 Aug 27;14(8):e0221164. doi: 10.1371/journal.pone.0221164. eCollection 2019. PubMed Article
Yoshimatsu S, Sone T, Nakajima M, Sato T, Okochi R, Ishikawa M, Nakamura M, Sasaki E, Shiozawa S, Okano H
PLoS One. 2019 Aug 27;14(8):e0221164. doi: 10.1371/journal.pone.0221164. eCollection 2019. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 141005 | pENTR-L1-PGK-PuroTK-L2 | Mutisite Gateway mediated vector construction |
| 141006 | pENTR-L1-PGK-HygTK-L2 | Mutisite Gateway mediated vector construction |
| 141007 | pENTR-L1-PGK-Neo-L2 | Mutisite Gateway mediated vector construction |
| 141008 | pENTR-L1-PGK-TKNeo-L2 | Mutisite Gateway mediated vector construction |
| 141009 | pENTR-L1-PGK-TKBsd-L2 | Mutisite Gateway mediated vector construction |
| 141010 | pUC-DEST-R3R4(R) | Mutisite Gateway mediated vector construction |
| 141011 | pUC-DTA-DEST-R3R4(R) | Mutisite Gateway mediated vector construction |
| 141012 | pATD | Mutisite Gateway mediated vector construction |
| 141013 | pDEST-Cas9-tdTomato | Mutisite Gateway mediated vector construction |
| 141014 | pDONR-P3P1r | Mutisite Gateway mediated vector construction |
| 141015 | pDONR-P2rP4 | Mutisite Gateway mediated vector construction |
| 141016 | pENTR2-L3-PBL-R1 | Mutisite Gateway mediated vector construction |
| 141017 | pENTR2-R2-PBR-L4 | Mutisite Gateway mediated vector construction |
| 141018 | pENTR2-L3-SfoI-EGFP-PBL-R1 | Mutisite Gateway mediated vector construction |
| 141019 | pENTR2-L3-SfoI-Venus-PBL-R1 | Mutisite Gateway mediated vector construction |
| 141020 | pENTR2-L3-SfoI-Cerulean-PBL-R1 | Mutisite Gateway mediated vector construction |
| 141021 | pENTR2-L3-SfoI-tdTomato-PBL-R1 | Mutisite Gateway mediated vector construction |
| 141022 | pENTR2-L3-SfoI-mCherry-PBL-R1 | Mutisite Gateway mediated vector construction |
| 141023 | pENTR2-L3-SfoI-iRFP713-PBL-R1 | Mutisite Gateway mediated vector construction |
| 141024 | pENTR2-L3-SfoI-IRES-EGFP-PBL-R1 | Mutisite Gateway mediated vector construction |
| 141025 | pENTR2-L3-SfoI-EGFP-secNanoLuc-PBL-R1 | Mutisite Gateway mediated vector construction |
| 141026 | pUC-DEST-hSOX2-EGFP-PuroTK | Reporter integration |
| 141027 | pUC-DEST-hPROX1-tdTomato-PuroTK | Reporter integration |
| 141028 | pUC-DEST-cjBLIMP1-Venus-HygTK | Reporter integration |
| 141029 | pUC-DEST-cjSTELLA-mCerulean-PuroTK | Reporter integration |
| 141030 | pPB-VASA-tdTomato-pNeo | Reporter integration |
| 141031 | pENTR-L1-U6-BbsI-R5 | Mutisite Gateway mediated vector construction |
| 141032 | pENTR-L1-U6-BsmBI-R5 | Mutisite Gateway mediated vector construction |
| 141033 | pENTR-R4-H1-BbsI-L2 | Mutisite Gateway mediated vector construction |
| 141034 | pENTR-R4-H1-BsmBI-L2 | Mutisite Gateway mediated vector construction |
| 141035 | pENTR-L5-CMV-R3 | Mutisite Gateway mediated vector construction |
| 141036 | pENTR-L5-CAG-R3 | Mutisite Gateway mediated vector construction |
| 141037 | pENTR-L5-EF1_-R3 | Mutisite Gateway mediated vector construction |
| 141038 | pENTR-L3-Cas9-L2 | Mutisite Gateway mediated vector construction |
| 141039 | pENTR-L3-Cas9n-L2 | Mutisite Gateway mediated vector construction |
| 141040 | pENTR-L3-Csy4-Cas9-L2 | Mutisite Gateway mediated vector construction |
| 141041 | pENTR-L3-Csy4-Cas9n-L2 | Mutisite Gateway mediated vector construction |
| 141042 | pENTR-L3-Csy4-FokI-dCas9-L2 | Mutisite Gateway mediated vector construction |
| 141043 | pENTR-L3-Cas9-EGFP-L2 | Mutisite Gateway mediated vector construction |
| 141044 | pENTR-L3-Cas9n-EGFP-L2 | Mutisite Gateway mediated vector construction |
| 141045 | pENTR-L3-Csy4-Cas9-EGFP-L2 | Mutisite Gateway mediated vector construction |
| 141046 | pENTR-L3-Csy4-Cas9n-EGFP-L2 | Mutisite Gateway mediated vector construction |
| 141047 | pENTR-L3-Csy4-FokI-dCas9-EGFP-L2 | Mutisite Gateway mediated vector construction |
| 141048 | pENTR-L3-SpCas9-HF1-2A-EGFP-L2 | Mutisite Gateway mediated vector construction |
| 141049 | pENTR-L3-SpCas9-HF4-2A-EGFP-L2 | Mutisite Gateway mediated vector construction |
| 141050 | pENTR-L3-SpCas9-HF2-2A-EGFP-L2 | Mutisite Gateway mediated vector construction |
| 141051 | pENTR-L3-eSpCas9(1.1)-2A-EGFP-L2 | Mutisite Gateway mediated vector construction |
| 141052 | pENTR-L3-eSpCas9n(1.1)-2A-EGFP-L2 | Mutisite Gateway mediated vector construction |
| 141053 | CSIV-DEST | Mutisite Gateway mediated vector construction |
| 141054 | pUC-DEST-R1R4 | Mutisite Gateway mediated vector construction |
| 141055 | pUC-DEST-R5R4 | Mutisite Gateway mediated vector construction |
| 141056 | pUC-DEST-R1R2 | Mutisite Gateway mediated vector construction |
| 141057 | pPB-DEST-R1R2-pNeo | Mutisite Gateway mediated vector construction |
| 141058 | pPB-DEST-R5R4-pNeo | Mutisite Gateway mediated vector construction |
| 141059 | pPB-DEST-R5R4-pHygTK | Mutisite Gateway mediated vector construction |
| 141060 | pPB-DEST-R5R4-pPuroTK | Mutisite Gateway mediated vector construction |
| 141061 | pENTR-R2L4 | Mutisite Gateway mediated vector construction |
| 141062 | pENTR-R2-pA-L4 | Mutisite Gateway mediated vector construction |
| 141063 | pENTR-R2-WPRE-pA-L4 | Mutisite Gateway mediated vector construction |
| 141064 | pDONR-P5P4 | Mutisite Gateway mediated vector construction |