Structure-based engineering of anti-GFP nanobody tandems as ultra-high-affinity reagents for purification.
Zhang Z, Wang Y, Ding Y, Hattori M
Sci Rep. 2020 Apr 10;10(1):6239. doi: 10.1038/s41598-020-62606-7. PubMed Article
Zhang Z, Wang Y, Ding Y, Hattori M
Sci Rep. 2020 Apr 10;10(1):6239. doi: 10.1038/s41598-020-62606-7. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 140442 | N8his-GFPenhancer-GGGGS4-LaG16 | We designed a flexible linker to connect two types of anti GFP nanobodies and used this fusion nanobody to purify GFP tagged protein. |