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Engineering human ACE2 to optimize binding to the spike protein of SARS coronavirus 2.
Chan KK, Dorosky D, Sharma P, Abbasi SA, Dye JM, Kranz DM, Herbert AS, Procko E
Science. 2020 Sep 4;369(6508):1261-1265. doi: 10.1126/science.abc0870. Epub 2020 Aug 4.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
141183pcDNA3-SARS-CoV-2-S-RBD-FcMammalian expression plasmid for RBD of SARS-CoV-2 protein S (spike) fused with IgG1-Fc
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141184pcDNA3-SARS-CoV-2-S-RBD-sfGFPMammalian expression plasmid for RBD of SARS-CoV-2 protein S (spike) fused with sfGFP
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141185pCEP4-myc-ACE2Mammalian expression plasmid for human ACE2 with an N-terminal (extracellular) c-myc tag
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145145pcDNA3-SARS-CoV-2-S-RBD-8hisMammalian expression plasmid for RBD of SARS-CoV-2 protein S (spike) with 8his tag
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145146pcDNA3-J chainMammalian expression plasmid for J chain of polymeric antibody classes
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145147pcDNA3-sACE2(WT)Mammalian expression plasmid for soluble ACE2 (protease domain)
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145148pcDNA3-sACE2v1Mammalian expression plasmid for soluble ACE2 (protease domain) high affinity variant 1
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145149pcDNA3-sACE2v2Mammalian expression plasmid for soluble ACE2 (protease domain) high affinity variant 2
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145150pcDNA3-sACE2v3Mammalian expression plasmid for soluble ACE2 (protease domain) high affinity variant 3
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145151pcDNA3-sACE2v4Mammalian expression plasmid for soluble ACE2 (protease domain) high affinity variant 4
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145152pcDNA3-sACE2v5Mammalian expression plasmid for soluble ACE2 (protease domain) high affinity variant 5
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145153pcDNA3-sACE2v6Mammalian expression plasmid for soluble ACE2 (protease domain) high affinity variant 6
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145154pcDNA3-sACE2(WT)-Fc(IgA1)Mammalian expression plasmid for soluble ACE2-Fc fusion (IgA1)
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145155pcDNA3-sACE2v1-Fc(IgA1)Mammalian expression plasmid for soluble ACE2-Fc fusion (IgA1) high affinity variant 1
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145156pcDNA3-sACE2-T92QMammalian expression plasmid for soluble ACE2 (protease domain) T92Q glycosylation mutant
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145157pcDNA3-sACE2v2-Fc(IgA1)Mammalian expression plasmid for soluble ACE2-Fc fusion (IgA1) high affinity variant 2
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145158pcDNA3-sACE2v3-Fc(IgA1)Mammalian expression plasmid for soluble ACE2-Fc fusion (IgA1) high affinity variant 3
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145159pcDNA3-sACE2v4-Fc(IgA1)Mammalian expression plasmid for soluble ACE2-Fc fusion (IgA1) high affinity variant 4
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145160pcDNA3-sACE2v5-Fc(IgA1)Mammalian expression plasmid for soluble ACE2-Fc fusion (IgA1) high affinity variant 5
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145161pcDNA3-sACE2v6-Fc(IgA1)Mammalian expression plasmid for soluble ACE2-Fc fusion (IgA1) high affinity variant 6
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145162pcDNA3-sACE2-T92Q-Fc(IgA1)Mammalian expression plasmid for soluble ACE2-Fc fusion (IgA1) glycosylation mutant T92Q
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145163pcDNA3-sACE2(WT)-Fc(IgG1)Mammalian expression plasmid for soluble ACE2-Fc fusion (IgG1)
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145164pcDNA3-sACE2v1-Fc(IgG1)Mammalian expression plasmid for soluble ACE2-Fc fusion (IgG1) high affinity variant 1
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145165pcDNA3-sACE2v2-Fc(IgG1)Mammalian expression plasmid for soluble ACE2-Fc fusion (IgG1) high affinity 2
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145166pcDNA3-sACE2v3-Fc(IgG1)Mammalian expression plasmid for soluble ACE2-Fc fusion (IgG1) high affinity 3
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145167pcDNA3-sACE2v4-Fc(IgG1)Mammalian expression plasmid for soluble ACE2-Fc fusion (IgG1) high affinity 4
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145168pcDNA3-sACE2v5-Fc(IgG1)Mammalian expression plasmid for soluble ACE2-Fc fusion (IgG1) high affinity 5
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145169pcDNA3-sACE2v6-Fc(IgG1)Mammalian expression plasmid for soluble ACE2-Fc fusion (IgG1) high affinity 6
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145170pcDNA3-sACE2-T92Q-Fc(IgG1)Mammalian expression plasmid for soluble ACE2-Fc fusion (IgG1) glycosylation mutant T92Q
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145171pcDNA3-sACE2(WT)-sfGFPMammalian expression plasmid for soluble ACE2-super folder GFP fusion
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145172pcDNA3-sACE2v1-sfGFPMammalian expression plasmid for sACE2-sfGFP high affinity variant 1
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145173pcDNA3-sACE2v2-sfGFPMammalian expression plasmid for sACE2-sfGFP high affinity variant 2
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145174pcDNA3-sACE2v3-sfGFPMammalian expression plasmid for sACE2-sfGFP high affinity variant 3
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145175pcDNA3-sACE2v4-sfGFPMammalian expression plasmid for sACE2-sfGFP high affinity variant 4
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145176pcDNA3-sACE2v5-sfGFPMammalian expression plasmid for sACE2-sfGFP high affinity variant 5
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145177pcDNA3-sACE2v6-sfGFPMammalian expression plasmid for sACE2-sfGFP high affinity variant 6
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145178pcDNA3-sACE2-T92Q-sfGFPMammalian expression plasmid for sACE2-sfGFP glycosylation mutant T92Q
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149268pcDNA3-sACE2(WT)-8hisMammalian expression plasmid for his-tagged sACE2
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149269pcDNA3-sACE2v1-8hisMammalian expression plasmid for his-tagged sACE2 high affinity variant 1
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149270pcDNA3-sACE2v2-8hisMammalian expression plasmid for his-tagged sACE2 high affinity variant 2
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149271pcDNA3-sACE2v3-8hisMammalian expression plasmid for his-tagged sACE2 high affinity variant 3
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149663pcDNA3-sACE2v2.2-8hisMammalian expression plasmid for his-tagged sACE2 high affinity variant 2.2
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149664pcDNA3-sACE2v2.4-8hisMammalian expression plasmid for his-tagged sACE2 high affinity variant 2.4
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149665pcDNA3-sACE2v2.2-sfGFPMammalian expression plasmid for sACE2-sfGFP high affinity variant 2.2
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149666pcDNA3-sACE2v2.4-sfGFPMammalian expression plasmid for sACE2-sfGFP high affinity variant 2.4
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149667pcDNA3-sACE2v2.2-IgG1Mammalian expression plasmid for soluble ACE2-Fc fusion (IgG1) high affinity 2.2
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149668pcDNA3-sACE2v2.4-IgG1Mammalian expression plasmid for soluble ACE2-Fc fusion (IgG1) high affinity 2.4
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154098pcDNA3-sACE2-WT(732)Mammalian expression plasmid for dimeric soluble ACE2 (untagged)
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154099pcDNA3-sACE2v2(732)Mammalian expression plasmid for dimeric soluble ACE2 high affinity variant 2 (untagged)
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154100pcDNA3-sACE2v2.4(732)Mammalian expression plasmid for dimeric soluble ACE2 high affinity variant 2.4 (untagged)
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154101pcDNA3-sACE2-WT(732)-8hMammalian expression plasmid for dimeric soluble ACE2 (8his tag)
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154102pcDNA3-sACE2v2(732)-8hMammalian expression plasmid for dimeric soluble ACE2 high affinity variant 2 (8his tag)
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154103pcDNA3-sACE2v2.4(732)-8hMammalian expression plasmid for dimeric soluble ACE2 high affinity variant 2.4 (8his tag)
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154104pcDNA3-sACE2-WT(732)-IgG1Mammalian expression plasmid for dimeric soluble ACE2 (IgG1-Fc fusion)
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154105pcDNA3-sACE2v2(732)-IgG1Mammalian expression plasmid for dimeric soluble ACE2 high affinity variant 2 (IgG1-Fc fusion)
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154106pcDNA3-sACE2v2.4(732)-IgG1Mammalian expression plasmid for dimeric soluble ACE2 high affinity variant 2.4 (IgG1-Fc fusion)
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