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Structural motifs in eIF4G and 4E-BPs modulate their binding to eIF4E to regulate translation initiation in yeast.
Gruner S, Weber R, Peter D, Chung MY, Igreja C, Valkov E, Izaurralde E
Nucleic Acids Res. 2018 Jul 27;46(13):6893-6908. doi: 10.1093/nar/gky542. PubMed Article

Plasmids from Article

ID Plasmid Purpose
148700pnEA-NpM-CteIF4G_954-1030_AFBacterial Expression of CteIF4G_954-1030
148705pnEA-NpM-CteIF4G_996-1033-GB1_AFBacterial Expression of CteIF4G_966-1033
148706pnEA-NpM-CteIF4G_996-1033-YLLtoAAA-GB1_AFBacterial Expression of CteIF4G_966-1033-YLLtoAAA
148707pnEA-NpM-ScEap1p_80-122_AFBacterial Expression of ScEap1p_80-122
148708pnEA-NpM-ScEap1p_80-150-GB1_AFBacterial Expression of ScEap1p_80-150
148709pnEA-NpM-ScEap1p_91-122-GB1_AFBacterial Expression of ScEap1p_91-122
148710pnEA-NpM-ScEap1p_91-150_AFBacterial Expression of ScEap1p_91-150
148711pnEA-NpM-ScEap1p_101-150-GB1_AFBacterial Expression of ScEap1p_101-150
148712pnEA-NpM-ScEap1p_107-122-GB1_AFBacterial Expression of ScEap1p_107-122
148713pnEA-NpM-SceIF4G_408-485_AFBacterial Expression of SceIF4G_408-485
148714pnEA-NpM-SceIF4G_408-500_AFBacterial Expression of SceIF4G_408-500
148715pnEA-NpM-SceIF4G_448-465-GB1_AFBacterial Expression of SceIF4G_448-465
148716pnEA-NpM-SceIF4G_448-500-GB1_AFBacterial Expression of SceIF4G_448-500
148717pnEA-NpM-Scp20_1-17-GB1_AFBacterial Expression of Scp20_1-17
148718pnEA-NpM-Scp20_1-49_AFBacterial Expression of Scp20_1-49
148719pnEA-NpM-Scp20_1-49-GB1_AFBacterial Expression of Scp20_1-49
148720pnEA-NpM-Scp20_18-49-GB1_AFBacterial Expression of Scp20_18-49
148721pnYC-CvH-CteIF4E_35-250-del183-202_AFBacterial Expression of CteIF4E_35-250-del183-202
148722pnYC-CvH-CteIF4E_35-250-del183-202-I87AI91A_AFBacterial Expression of CteIF4E_35-250-del183-202-I87AI91A
148723pnYC-CvH-CteIF4E_40-250-del183-202_AFBacterial Expression of CteIF4E_40-250-del183-202
148725pnYC-NpH-CteIF4E_35-250-del183-202_AFBacterial Expression of CteIF4E_35-250-del183-202
148726pnYC-NpH-SceIF4E_25-213_AFBacterial Expression of SceIF4E_25-213
148727pnYC-NpH-SceIF4E_25-213-I77AI81A_AFBacterial Expression of SceIF4E_25-213-I77AI81A
148728pnYC-NpH-SceIF4E_25-213-K42AK168AK187A_AFBacterial Expression of SceIF4E_25-213-K42AK168AK187A
148729pnYC-NpH-SceIF4E_25-213-KKKKtoAAAA_AFBacterial Expression of SceIF4E_25-213-KKKKtoAAAA
148730pnYC-NpH-SceIF4E_35-213_AFBacterial Expression of SceIF4E_35-213
148811pnEA-NpM-ScEap1p_101-122-GB1_AGBacterial Expression of ScEap1p_101-122
148812pnEA-NpM-ScEap1p_107-122-MNYHto IDFQ-GB1_AGBacterial Expression of ScEap1p_107-122-MNYHtoIDFQ
148813pnEA-NpM-ScEap1p_107-122-Y107IA108KS110T-GB1_AGBacterial Expression of ScEap1p_107-122-Y107IA108KS110T
148814pnEA-NpM-ScEap1p_123-150-GB1_AGBacterial Expression of ScEap1p_123-150

Antibodies from Article