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Addgene
Efficient Transgenesis in Caenorhabditis elegans Using Flp Recombinase-Mediated Cassette Exchange.
Nonet ML
Genetics. 2020 Jun 8. pii: genetics.120.303388. doi: 10.1534/genetics.120.303388.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
153083pLF3FShCRMCE integration vector with self excising cassette
153086pNM3732mec-4p GFP-C1 positive control for RMCE integration
154108pNM3749loxP FRT FRT3 RMCE landing site in a Golden Gate SapI GCG ACG assembly vector
154109pNM3746loxP FLP FRT FRT3 landing site in a Golden Gate SapI GCG ACG assembly vector
154110pNM3649miniMos dual component RMCE landing site vector
154111pNM3689miniMos single component RMCE landing site vector
154112pNM3724tetO 7X ∆pes-10 promoter driving GFP-C1 in an RCME vector
154113pNM3725UAS 11X ∆pes-10 promoter driving GFP-C1 in an RCME vector
154114pNM3726QUAS 5X ∆pes-10 promoter driving GFP-C1 in an RCME vector
154115pNM3727lexO 5X ∆pes-10 promoter driving GFP-C1 in an RCME vector
154116pNM3755tetO 7X ∆pes-10 promoter driving GFP-C1 & an act-4 3' UTR in a RMCE vector
154117pNM3774tetO 7X enhancer with ∆mec-7 basal promoter driving GFP-C1
154118pNM3734mec-4 promoter driving GAL4-QFAD in an RCME vector
154119pNM3810mec-4 promoter driving QF in an RCME vector
154120pNM3811mec-4 promoter driving QF2 in an RCME vector
154121pNM3813mec-4 promoter driving lexA-L-QFAD in an RCME vector
154122pNM3814mec-4 promoter driving tet ON rtetR-L-QFAD in an RCME vector
154123pNM3823mec-4 promoter driving tet OFF tetR-L-QFAD in an RCME vector

Antibodies from Article