Acoustic biosensors for ultrasound imaging of enzyme activity.
Lakshmanan A, Jin Z, Nety SP, Sawyer DP, Lee-Gosselin A, Malounda D, Swift MB, Maresca D, Shapiro MG
Nat Chem Biol. 2020 Sep;16(9):988-996. doi: 10.1038/s41589-020-0591-0. Epub 2020 Jul 13. PubMed Article
Lakshmanan A, Jin Z, Nety SP, Sawyer DP, Lee-Gosselin A, Malounda D, Swift MB, Maresca D, Shapiro MG
Nat Chem Biol. 2020 Sep;16(9):988-996. doi: 10.1038/s41589-020-0591-0. Epub 2020 Jul 13. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 153294 | WT N-His Ana GvpC in pET28a | Expresses N-terminal His-tagged GvpC from Anabaena flos-aquae in E.coli |
| 153295 | C-His-GvpC-Calpain | Expresses calpain-sensitive variant of Anabaena flos-aquae GvpC in E.coli |
| 153296 | C-His-GvpC-TEV | Expresses TEV-sensitive variant of Anabaena flos-aquae GvpC in E.coli |
| 153297 | N-His-GvpC-ssrA | Expresses ClpXP-degradable variant of Anabaena flos-aquae GvpC in E.coli |
| 153298 | pTetR-BCD2-Ana GvpC | Expresses wild-type gas vesicle protein C (GvpC) from Anabaena flos-aquae |
| 153299 | pET28a-T5-ASG_ClpXP | Expresses acoustic sensor genes or ClpXP-sensitive gas vesicles in E. coli Nissle 1917 |
| 153301 | araBAD-BCD20-ClpP-BCD17-ClpX | Expresses clpX and clpP in E.Coli |
| 153302 | pBEST_OR2-OR1-Pr-UTR1_ClpP-T500 | Expresses ClpP protease in the cell-free TX-TL system |