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High force catch bond mechanism of bacterial adhesion in the human gut.
Liu Z, Liu H, Vera AM, Bernardi RC, Tinnefeld P, Nash MA
Nat Commun. 2020 Aug 28;11(1):4321. doi: 10.1038/s41467-020-18063-x. PubMed Article

Plasmids from Article

ID Plasmid Purpose
153439pET28a-ybbr-ELP-ddFLN4-XMod-Doc-HIS (wild type)E. coli expression of Rc. XDocB wild-type construct containing ybbr tag, ELP linker and ddFLN4 fingerprint domain. This construct was designed for AFM measurements.
153440pET28a-ybbr-ELP-ddFLN4-XMod-Doc-HIS (BMA-KO)E. coli expression of Rc. XDocB binding mode A knock-out construct containing ybbr tag, ELP linker and ddFLN4 fingerprint domain. This construct was designed for AFM measurements.
153441pET28a-ybbr-ELP-ddFLN4-XMod-Doc-HIS (BMB-KO)E. coli expression of Rc. XDocB binding mode B knock-out construct containing ybbr tag, ELP linker and ddFLN4 fingerprint domain. This construct was designed for AFM measurements.
153442pET28a-ybbr-HIS-ELP-ddFLN4-I27-XMod-Doc (wild type)E. coli expression of Rc. XDocB wild-type construct containing ybbr tag, ELP linker and ddFLN4 and I27 fingerprint domains. This construct was designed for AFM measurements.
153443pET28a-ybbr-XMod-Doc (WT, S199AzF)-HISE. coli expression (amber suppression) of Rc. XDocB with serine at position 199 replaced with amber codon.
153444pET28a-ybbr-XMod-Doc (BMA-KO, S199AzF)-HISE. coli expression (amber suppression) of Rc. XDocB binding mode A knock-out mutant with serine at position 199 replaced with amber codon.
153445pET28a-ybbr-XMod-Doc (BMB-KO, S199AzF)-HISE. coli expression (amber suppression) of Rc. XDocB binding mode B knock-out mutant with serine at position 199 replaced with amber codon.
153446pET28a-Coh-ddFLN4-ELP-HIS-ybbrE. coli expression of Rc. CohE wild-type construct containing ybbr tag, ELP linker and ddFLN4 fingerprint domain. This construct was designed for AFM measurements.
153447pET28a-Avi-Coh (E154C)-HISE. coli expression of Rc. CohE construct with E154C mutation.

Antibodies from Article