Dihydroxyacetone phosphate signals glucose availability to mTORC1.
Orozco JM, Krawczyk PA, Scaria SM, Cangelosi AL, Chan SH, Kunchok T, Lewis CA, Sabatini DM
Nat Metab. 2020 Sep;2(9):893-901. doi: 10.1038/s42255-020-0250-5. Epub 2020 Jul 27. PubMed Article
Orozco JM, Krawczyk PA, Scaria SM, Cangelosi AL, Chan SH, Kunchok T, Lewis CA, Sabatini DM
Nat Metab. 2020 Sep;2(9):893-901. doi: 10.1038/s42255-020-0250-5. Epub 2020 Jul 27. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 154205 | HA-GLUT5 in pRK5 | N-terminal HA-tagged GLUT5 cDNA expression vector for transfections |
| 154206 | FLAG-TKFC in pLJM60 | Lentiviral vector for stable expression of TKFC |
| 154207 | FLAG-GK in PLJM60 | Lentiviral vector for stable expression of GK |
| 154209 | TPI in pCW57_tTA_Blast | Dox-off lentivirus construct all-in-one vector, with Blast resistance, for expression of TPI |
| 154210 | 3XFLAG_ALDOA in pCW57_tTA_Blast | Dox-off lentivirus construct all-in-one vector, with Blast resistance, for expression of ALDOA |
| 154211 | 3XFLAG_GAPDH in pCW57_tTA_Blast | Dox-off lentivirus construct all-in-one vector, with Blast resistance, for expression of GAPDH |
| 154212 | HA-mios in pRK5 | N-terminal HA-tagged Mios cDNA expression vector for transfections |
| 154213 | WDR24 in pRK5 | Untagged WDR24 cDNA expression vector for transfections |
| 154214 | WDR59 in pRK5 | Untagged WDR59 cDNA expression vector for transfections |
| 154215 | SEC13 in pRK5 | Untagged SEC13 cDNA expression vector for transfections |
| 154216 | SEH1L in pRK5 | Untagged SEH1L cDNA expression vector for transfections |
| 154217 | FLAG-TKGAPDH in pLJM60 | Lentiviral vector for stable expression of FLAG-TK-GAPDH |
| 154219 | HA_Axin1 in pRK5 | N-terminal HA-tagged AXIN1 cDNA expression vector for transfections |
| 154220 | Axin1_HA in pRK5 | C-terminal HA-tagged AXIN1 cDNA expression vector for transfections |