An expanded auxin-inducible degron toolkit for Caenorhabditis elegans.
Ashley GE, Duong T, Levenson MT, Martinez MAQ, Johnson LC, Hibshman JD, Saeger HN, Palmisano NJ, Doonan R, Martinez-Mendez R, Davidson BR, Zhang W, Ragle JM, Medwig-Kinney TN, Sirota SS, Goldstein B, Matus DQ, Dickinson DJ, Reiner DJ, Ward JD
Genetics. 2021 Mar 31;217(3). pii: 6104563. doi: 10.1093/genetics/iyab006. PubMed Article
Ashley GE, Duong T, Levenson MT, Martinez MAQ, Johnson LC, Hibshman JD, Saeger HN, Palmisano NJ, Doonan R, Martinez-Mendez R, Davidson BR, Zhang W, Ragle JM, Medwig-Kinney TN, Sirota SS, Goldstein B, Matus DQ, Dickinson DJ, Reiner DJ, Ward JD
Genetics. 2021 Mar 31;217(3). pii: 6104563. doi: 10.1093/genetics/iyab006. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 121054 | pJW1583 | GFP^SEC^AID*::3xFLAG vector with ccdB sites for cloning homology arms. |
| 121055 | pJW1584 | YPET^SEC^AID*::3xFLAG vector with ccdB sites for cloning homology arms. |
| 121057 | pJW1586 | mKate2^SEC^AID*::3xFLAG vector with ccdB sites for cloning homology arms. |
| 121059 | pJW1592 | GFP^SEC^BioTag::AID*::3xFLAG vector with ccdB sites for cloning homology arms. |
| 121061 | pJW1594 | TagRFP-T^SEC^BioTag::AID*::3xFLAG vector with ccdB sites for cloning homology arms. |
| 121062 | pJW1595 | mKate2^SEC^BioTag::AID*::3xFLAG vector with ccdB sites for cloning homology arms. |
| 154310 | pJW1347 | Donor-cassette for SapTrap |
| 154311 | pJW1348 | NT Donor-cassette for SapTrap |
| 154312 | pJW1582 | mNeonGreen^SEC^AID*::3xFLAG vector with ccdB sites for cloning homology arms. |
| 154314 | pJW1659 | NT Donor-cassette for SapTrap |
| 154315 | pJW1759 | NT Donor-cassette for SapTrap |
| 154316 | pJW1787 | NT Donor-cassette for SapTrap |
| 154317 | pJW1788 | CT Donor-cassette for SapTrap |
| 154318 | pJW1805 | CT Donor-cassette for SapTrap |
| 154319 | pJW1806 | NT Donor-cassette for SapTrap |
| 154320 | pJW1816 | Multi-cassette for SapTrap |
| 154321 | pJW1820 | Multi-cassette for SapTrap |
| 154322 | pJW1822 | Multi-cassette for SapTrap |
| 154323 | pJW1827 | Multi-cassette for SapTrap |
| 154324 | pJW1838 | sgRNA Destination Vector for SapTrap |
| 154325 | pJW1839 | sgRNA Destination Vector for SapTrap |
| 154326 | pJW1846 | Multi-cassette for SapTrap |
| 154327 | pJW1849 | sgRNA Target Vector for CRISPR |
| 154328 | pJW1850 | sgRNA Target Vector for CRISPR |
| 154329 | pJW1851 | sgRNA Target Vector for CRISPR |
| 154330 | pJW1882 | sgRNA Target Vector for CRISPR |
| 154331 | pJW1883 | sgRNA Target Vector for CRISPR |
| 154332 | pJW1884 | sgRNA Target Vector for CRISPR |
| 154333 | pJW1886 | Multi-cassette for SapTrap |
| 154334 | pJW2072 | Crispr repair template: add insertion site specific homology arms by PCR before insertion |
| 154335 | pJW2086 | Crispr repair template: add insertion site specific homology arms by PCR before insertion |
| 154336 | pJW2088 | Crispr repair template: add insertion site specific homology arms by PCR before insertion |
| 154337 | pJW1836 | Promoter reporter |
| 154338 | pJW1841 | Promoter reporter |
| 154339 | pJW1947 | Enhancer reporter |
| 154340 | pJW1948 | Enhancer reporter |
| 163092 | pJW1821 | CRISPR repair template: add insertion site specific homology arms by PCR before insertion |
| 163093 | pJW1927 | CRISPR repair template: add insertion site specific homology arms by PCR before insertion |
| 163094 | pJW2098 | CRISPR repair template: add insertion site specific homology arms by PCR before insertion |
| 163095 | pJW2171 | CRISPR repair template: add insertion site specific homology arms by PCR before insertion |
| 163096 | pJW2172 | CRISPR repair template: add insertion site specific homology arms by PCR before insertion |