An expanded auxin-inducible degron toolkit for Caenorhabditis elegans
Ashley G, Duong T, Levenson MT, Martinez MAQ, Johnsen LC, Hibshman JD, Saeger HN, Palmisano NJ, Doonan R, Martinez-Mendez R, Davidson B, Zhang W, Ragle JM, Medwig-Kinney TN, Sirota SS, Goldstein B, Matus DQ, Dickinson DJ, Reiner DJ, Ward JD
Genetics, 2021 Article
Ashley G, Duong T, Levenson MT, Martinez MAQ, Johnsen LC, Hibshman JD, Saeger HN, Palmisano NJ, Doonan R, Martinez-Mendez R, Davidson B, Zhang W, Ragle JM, Medwig-Kinney TN, Sirota SS, Goldstein B, Matus DQ, Dickinson DJ, Reiner DJ, Ward JD
Genetics, 2021 Article
Plasmids from Article
| ID | Plasmid | Purpose | |
|---|---|---|---|
| 121054 | pJW1583 | GFP^SEC^AID*::3xFLAG vector with ccdB sites for cloning homology arms. |
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| 121055 | pJW1584 | YPET^SEC^AID*::3xFLAG vector with ccdB sites for cloning homology arms. |
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| 121057 | pJW1586 | mKate2^SEC^AID*::3xFLAG vector with ccdB sites for cloning homology arms. |
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| 121059 | pJW1592 | GFP^SEC^BioTag::AID*::3xFLAG vector with ccdB sites for cloning homology arms. |
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| 121061 | pJW1594 | TagRFP-T^SEC^BioTag::AID*::3xFLAG vector with ccdB sites for cloning homology arms. |
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| 121062 | pJW1595 | mKate2^SEC^BioTag::AID*::3xFLAG vector with ccdB sites for cloning homology arms. |
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| 154310 | pJW1347 | Donor-cassette for SapTrap |
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| 154311 | pJW1348 | NT Donor-cassette for SapTrap |
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| 154312 | pJW1582 | mNeonGreen^SEC^AID*::3xFLAG vector with ccdB sites for cloning homology arms. |
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| 154314 | pJW1659 | NT Donor-cassette for SapTrap |
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| 154315 | pJW1759 | NT Donor-cassette for SapTrap |
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| 154316 | pJW1787 | NT Donor-cassette for SapTrap |
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| 154317 | pJW1788 | CT Donor-cassette for SapTrap |
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| 154318 | pJW1805 | CT Donor-cassette for SapTrap |
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| 154319 | pJW1806 | NT Donor-cassette for SapTrap |
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| 154320 | pJW1816 | Multi-cassette for SapTrap |
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| 154321 | pJW1820 | Multi-cassette for SapTrap |
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| 154322 | pJW1822 | Multi-cassette for SapTrap |
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| 154323 | pJW1827 | Multi-cassette for SapTrap |
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| 154324 | pJW1838 | sgRNA Destination Vector for SapTrap |
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| 154325 | pJW1839 | sgRNA Destination Vector for SapTrap |
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| 154326 | pJW1846 | Multi-cassette for SapTrap |
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| 154327 | pJW1849 | sgRNA Target Vector for CRISPR |
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| 154328 | pJW1850 | sgRNA Target Vector for CRISPR |
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| 154329 | pJW1851 | sgRNA Target Vector for CRISPR |
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| 154330 | pJW1882 | sgRNA Target Vector for CRISPR |
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| 154331 | pJW1883 | sgRNA Target Vector for CRISPR |
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| 154332 | pJW1884 | sgRNA Target Vector for CRISPR |
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| 154333 | pJW1886 | Multi-cassette for SapTrap |
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| 154334 | pJW2072 | Crispr repair template: add insertion site specific homology arms by PCR before insertion |
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| 154335 | pJW2086 | Crispr repair template: add insertion site specific homology arms by PCR before insertion |
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| 154336 | pJW2088 | Crispr repair template: add insertion site specific homology arms by PCR before insertion |
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| 154337 | pJW1836 | Promoter reporter |
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| 154338 | pJW1841 | Promoter reporter |
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| 154339 | pJW1947 | Enhancer reporter |
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| 154340 | pJW1948 | Enhancer reporter |
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| 163092 | pJW1821 | CRISPR repair template: add insertion site specific homology arms by PCR before insertion |
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| 163093 | pJW1927 | CRISPR repair template: add insertion site specific homology arms by PCR before insertion |
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| 163094 | pJW2098 | CRISPR repair template: add insertion site specific homology arms by PCR before insertion |
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| 163095 | pJW2171 | CRISPR repair template: add insertion site specific homology arms by PCR before insertion |
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| 163096 | pJW2172 | CRISPR repair template: add insertion site specific homology arms by PCR before insertion |
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