Conditional Single Vector CRISPR/SaCas9 Viruses for Efficient Mutagenesis in the Adult Mouse Nervous System.
Hunker AC, Soden ME, Krayushkina D, Heymann G, Awatramani R, Zweifel LS
Cell Rep. 2020 Mar 24;30(12):4303-4316.e6. doi: 10.1016/j.celrep.2020.02.092. PubMed Article
Hunker AC, Soden ME, Krayushkina D, Heymann G, Awatramani R, Zweifel LS
Cell Rep. 2020 Mar 24;30(12):4303-4316.e6. doi: 10.1016/j.celrep.2020.02.092. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 124844 | pAAV-FLEX-SaCas9-U6-sgRNA | Vector for Cre-dependent expression of SaCas9 |
| 124845 | pAAV-FLEXfrt-SaCas9-U6-sgRNA | Vector for FLP-dependent expression of SaCas9 with gRNA |
| 124846 | pAAV-FLEX-SaCas9-U6-sgSlc17a7 | Mutagenesis of Slc17a7 |
| 124847 | pAAV-FLEX-SaCas9-U6-sgSlc17a6 | Mutagenesis of Slc17a6 with SauCas9 |
| 124848 | pAAV-FLEX-SaCas9-U6-sgSlc17a8 | Mutagenesis of Slc17a8 |
| 124849 | pAAV-FLEX-SaCas9-U6-sgSlc18a2 | Mutagenesis of Slc18a2 |
| 124850 | pAAV-FLEX-SaCas9-U6-sgGrin2a | Mutagenesis of Grin2a |
| 124851 | pAAV-FLEX-SaCas9-U6-sgGrin2b | Mutagenesis of Grin2b |
| 124852 | pAAV-FLEX-SaCas9-U6-sgGrin1 | Mutagenesis of Grin1 |
| 124853 | pAAV-FLEX-SaCas9-U6-sgGria1 | Mutagenesis of Gria1 |
| 124854 | pAAV-FLEX-SaCas9-U6-sgGria2 | Mutagenesis of Gria2 |
| 124855 | pAAV-FLEX-SaCas9-U6-sgGria3 | Mutagenesis of Gria3 |
| 124856 | pAAV-FLEX-SaCas9-U6-sgGabrg1 | Mutagenesis of Gabrg1 |
| 124857 | pAAV-FLEX-SaCas9-U6-sgGabrg2 | Mutagenesis of Gabrg2 |
| 124858 | pAAV-FLEX-SaCas9-U6-sgGabrg3 | Mutagenesis of Gabrg3 |
| 124859 | pAAV-FLEXfrt-SaCas9-U6-sgSlc17a7 | Mutagenesis of Slc17a7 |
| 124860 | pAAV-FLEXfrt-SaCas9-U6-sgSlc17a6 | Mutagenesis of Slc17a6 |
| 124861 | pAAV-FLEXfrt-SaCas9-U6-sgSlc17a8 | Mutagenesis of Slc17a8 |
| 124862 | pAAV-FLEXfrt-SaCas9-U6-sgSlc18a2 | Mutagenesis of Slc18a2 |
| 124863 | pAAV-FLEXfrt-SaCas9-U6-sgGrin2a | Mutagenesis of Grin2a |
| 124864 | pAAV-FLEXfrt-SaCas9-U6-sgGrin2b | Mutagenesis of Grin2b |
| 124865 | pAAV-FLEXfrt-SaCas9-U6-sgGrin1 | Mutagenesis of Grin1 |
| 124867 | pAAV-FLEXfrt-SaCas9-U6-sgGria1 | Mutagenesis of Gria1 |
| 124868 | pAAV-FLEXfrt-SaCas9-U6-sgGria2 | Mutagenesis of Gria2 |
| 124869 | pAAV-FLEXfrt-SaCas9-U6-sgGria3 | Mutagenesis of Gria3 |
| 124870 | pAAV-FLEXfrt-SaCas9-U6-sgGabrg1 | Mutagenesis of Gabrg1 |
| 124871 | pAAV-FLEXfrt-SaCas9-U6-sgGabrg2 | Mutagenesis of Gabrg2 |
| 124872 | pAAV-FLEXfrt-SaCas9-U6-sgGabrg3 | Mutagenesis of Gabrg3 |
| 154373 | pAAV-FLEX-EGFP-KASH | Vector for Cre-dependent expression of EGFP tagged KASH protein domain |
| 154374 | pAAV-FLEXfrt-EGFP-KASH | Vector for Flp-dependent expression of EGFP tagged KASH protein domain |