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Justin Kollman Lab: Plasmids for the Expression and Co-Expression of COVID-19 Proteins Nsp10 and Nsp14
Unpublished

Plasmids from Article

ID Plasmid Purpose  
154952pET28-Nsp14-TEV-6HisExpresses Nsp14 from SARS-CoV-2 with a C-terminal TEV-protease cleavable 6His tag in E. coli
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154953pET28-Nsp14-TEV-StrepExpresses Nsp14 from SARS-CoV-2 with a C-terminal TEV-protease cleavable Strep tag in E. coli
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154954pET28-6His-TEV-Nsp14Expresses Nsp14 from SARS-CoV-2 with a N-terminal TEV-protease cleavable 6His tag in E. coli
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154955pET28-Strep-TEV-Nsp14Expresses Nsp14 from SARS-CoV-2 with a N-terminal TEV-protease cleavable Strep tag in E. coli
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154956pET28-6His-SUMO-Nsp14Expresses Nsp14 from SARS-CoV-2 with a N-terminal SUMO-protease cleavable 6His-SUMO tag in E. coli
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154957pET22-Nsp10-TEV-6HisExpresses Nsp10 from SARS-CoV-2 with a C-terminal TEV-protease cleavable 6His tag in E. coli
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154958pET22-Strep-TEV-Nsp10Expresses Nsp10 from SARS-CoV-2 with a N-terminal TEV-protease cleavable Strep tag in E. coli
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154959pET28-6His-SUMO-Nsp10Expresses Nsp10 from SARS-CoV-2 with a N-terminal SUMO-protease cleavable 6His-SUMO tag in E. coli
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154960pET22-6His-SUMO-Nsp10Expresses Nsp10 from SARS-CoV-2 with a N-terminal SUMO-protease cleavable 6His-SUMO tag in E. coli
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