Genetic interaction mapping and exon-resolution functional genomics with a hybrid Cas9-Cas12a platform.
Gonatopoulos-Pournatzis T, Aregger M, Brown KR, Farhangmehr S, Braunschweig U, Ward HN, Ha KCH, Weiss A, Billmann M, Durbic T, Myers CL, Blencowe BJ, Moffat J
Nat Biotechnol. 2020 May;38(5):638-648. doi: 10.1038/s41587-020-0437-z. Epub 2020 Mar 16. PubMed Article
Gonatopoulos-Pournatzis T, Aregger M, Brown KR, Farhangmehr S, Braunschweig U, Ward HN, Ha KCH, Weiss A, Billmann M, Durbic T, Myers CL, Blencowe BJ, Moffat J
Nat Biotechnol. 2020 May;38(5):638-648. doi: 10.1038/s41587-020-0437-z. Epub 2020 Mar 16. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 155046 | plenti-Lb-Cas12a-2xNLS | Lentiviral vector expressing human codon-optimized _C-terminal Myc-tagged Lachnospiraceae bacterium (Lb)-Cas12a nuclease with N- and C-terminal NLS and Neomycin/G418/Geneticin resistance from EF-1alpha promoter |
| 155047 | plenti-As-Cas12a-2xNLS | Lentiviral vector expressing human codon-optimized _C-terminal Myc-tagged _Acidaminococcus sp. BV3L6 (As)-Cas12a nuclease with N- and C-terminal NLS and Neomycin/G418/Geneticin resistance from EF-1alpha promoter |
| 155048 | pLCHKO | Lentiviral backbone for cloning and expressing U6 driven hybrid guide (hg)RNAs with BfuAI cloning sites and puromycin selection. |
| 155049 | Topo_SpCas9.tracr_LbCas12a.DR | TOPO vector for the cloning of _the SpCas9 tracrRNA - LbCas12a Direct Repeat (DR) fragment into the pLCHKO hgRNA vector |
| 155050 | Topo_SpCas9.tracr_AsCas12a.DR | TOPO vector for the cloning of _the SpCas9 tracrRNA - AsCas12a Direct Repeat (DR) fragment into the pLCHKO hgRNA vector |
| 155051 | pLCHKO_HPRT1_exon2_1_Lb | Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of HPRT1 exon 2 using SpCas9 and LbCas12a nucleases (CHyMErA system) |
| 155052 | pLCHKO_HPRT1_exon2_2_Lb | Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of HPRT1 exon 2 using SpCas9 and LbCas12a nucleases (CHyMErA system) |
| 155053 | pLCHKO_HPRT1_exon3_1_Lb | Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of HPRT1 exon 3 using SpCas9 and LbCas12a nucleases (CHyMErA system) |
| 155054 | pLCHKO_HPRT1_exon3_2_Lb | Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of HPRT1 exon 3 using SpCas9 and LbCas12a nucleases (CHyMErA system) |
| 155055 | pLCHKO_HPRT1_exon2_1_As | Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of HPRT1 exon 2 using SpCas9 and AsCas12a nucleases (CHyMErA system) |
| 155056 | pLCHKO_HPRT1_exon2_2_As | Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of HPRT1 exon 2 using SpCas9 and AsCas12a nucleases (CHyMErA system) |
| 155057 | pLCHKO_HPRT1_exon3_1_As | Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of HPRT1 exon 3 using SpCas9 and AsCas12a nucleases (CHyMErA system) |
| 155058 | pLCHKO_HPRT1_exon3_2_As | Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of HPRT1 exon 3 using SpCas9 and AsCas12a nucleases (CHyMErA system) |
| 155059 | pLCHKO_TK1_HPRT1_Lb_1 | Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA targeting TK1 using SpCas9 and HPRT1 using LbCas12a (CHyMErA system) |
| 155060 | pLCHKO_TK1_HPRT1_Lb_2 | Lentiviral plasmid for expressing U6 driven multiplexed hybrid guide (hg)RNA targeting TK1 using SpCas9 and an intergenic site & HPRT1 using LbCas12a (CHyMErA system) |
| 155061 | pLCHKO_TK1_HPRT1_Lb_3 | Lentiviral plasmid for expressing U6 driven multiplexed hybrid guide (hg)RNA targeting TK1 using SpCas9 and two intergenic sites & HPRT1 using LbCas12a (CHyMErA system) |
| 155062 | pLCHKO_TK1_HPRT1_Lb_4 | Lentiviral plasmid for expressing U6 driven multiplexed hybrid guide (hg)RNA targeting TK1 using SpCas9 and three intergenic sites & HPRT1 using LbCas12a (CHyMErA system) |
| 155063 | pLCHKO_TK1_HPRT1_Lb_5 | Lentiviral plasmid for expressing U6 driven multiplexed hybrid guide (hg)RNA targeting TK1 using SpCas9 and four intergenic sites & HPRT1 using LbCas12a (CHyMErA system) |
| 155064 | pLCHKO_TK1_HPRT1_Lb_6 | Lentiviral plasmid for expressing U6 driven multiplexed hybrid guide (hg)RNA targeting TK1 using SpCas9 and five intergenic sites & HPRT1 using LbCas12a (CHyMErA system) |
| 155065 | pLCHKO_PDPR_exon_deletion_1 | Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of PDPR exon using SpCas9 and LbCas12a nucleases (CHyMErA system) |
| 155066 | pLCHKO_PDPR_exon_deletion_2 | Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of PDPR exon using SpCas9 and LbCas12a nucleases (CHyMErA system) |
| 155067 | pLCHKO_PDPR_exon_deletion_3 | Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of PDPR exon using SpCas9 and LbCas12a nucleases (CHyMErA system) |
| 155068 | pLCHKO_PDPR_exon_deletion_4 | Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of PDPR exon using SpCas9 and LbCas12a nucleases (CHyMErA system) |
| 155069 | pLCHKO_SFRS7_exon_deletion_1 | Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of SFRS7 exon using SpCas9 and LbCas12a nucleases (CHyMErA system) |
| 155070 | pLCHKO_SFRS7_exon_deletion_2 | Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of SFRS7 exon using SpCas9 and LbCas12a nucleases (CHyMErA system) |
| 155071 | pLCHKO_SFRS7_exon_deletion_3 | Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of SFRS7 exon using SpCas9 and LbCas12a nucleases (CHyMErA system) |
| 155072 | pLCHKO_SFRS7_exon_deletion_4 | Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of SFRS7 exon using SpCas9 and LbCas12a nucleases (CHyMErA system) |
| 155073 | pLCHKO_MDM4_exon_deletion_1 | Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of MDM4 exon using SpCas9 and LbCas12a nucleases (CHyMErA system) |
| 155074 | pLCHKO_MDM4_exon_deletion_2 | Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of MDM4 exon using SpCas9 and LbCas12a nucleases (CHyMErA system) |
| 155075 | pLCHKO_MDM4_exon_deletion_3 | Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of MDM4 exon using SpCas9 and LbCas12a nucleases (CHyMErA system) |
| 155076 | pLCHKO_MDM4_exon_deletion_4 | Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of MDM4 exon using SpCas9 and LbCas12a nucleases (CHyMErA system) |
| 155077 | pLCHKO_intergenic-intergenic_1 | Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA targeting two intergenic sites in the human genome using SpCas9 and LbCas12a nucleases (CHyMErA system), respectively |
| 155078 | pLCHKO_intergenic-intergenic_2 | Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA targeting two intergenic sites in the human genome using SpCas9 and LbCas12a nucleases (CHyMErA system), respectively |
| 155079 | pLCHKO_GFP_Luciferase | Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA targeting GFP and Luciferase using SpCas9 and LbCas12a nucleases (CHyMErA system), respectively |
| 155081 | pLCHKO_Ptbp_ex8_1 | Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of mouse Ptbp exon 8 using SpCas9 and LbCas12a nucleases (CHyMErA system) |
| 155082 | pLCHKO_Ptbp_ex8_2 | Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of mouse Ptbp exon 8 using SpCas9 and LbCas12a nucleases (CHyMErA system) |
| 155083 | pLCHKO_Ptbp_ex8_3 | Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of mouse Ptbp exon 8 using SpCas9 and LbCas12a nucleases (CHyMErA system) |
| 155084 | pLCHKO_Ptbp_ex8_4 | Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of mouse Ptbp exon 8 using SpCas9 and LbCas12a nucleases (CHyMErA system) |
| 155199 | CHyMErA Paralog&Dual-targeting hgRNA Library | Hybrid guide RNA library targetings human paralog pairs and other protein-coding genes. |
| 155200 | CHyMErA Exon-deletion hgRNA Library | Hybrid guide RNA library targeting human alternative cassette exons for deletion and disruption |