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A microscopy-based kinetic analysis of yeast vacuolar protein sorting.
Casler JC, Glick BS
Elife. 2020 Jun 25;9. pii: 56844. doi: 10.7554/eLife.56844. PubMed Article

Plasmids from Article

ID Plasmid Purpose
140149YIplac211-Vph1-HaloTagPlasmid for pop-in/pop-out replacement of Vph1 with Vph1-HaloTag
140150YIplac211-GGA2-HaloTagx2Plasmid for pop-in/pop-out replacement of Gga2 with Gga2-HaloTagx2
140151YIplac211-Sec7-mScarlet2Ix6Plasmid for pop-in/pop-out replacement of Sec7 with Sec7-mScarlet2Ix6
140152YIplac211-Mup1-mScarlet2IPlasmid for pop-in/pop-out replacement of Mup1 with Mup1-mScarlet2I
140153YIplac204-pOst1-APVNTT-DsRed-Express2-FKBP(LV)C22V-QRPLExpresses a regulatable vacuolar cargo for yeast at the TRP1 locus
140154YIplac204-pOst1-ESCargo(Crimson)Expresses a vacuolar cargo for yeast at the TRP1 locus

Antibodies from Article