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CRISPR-Act3.0 for highly efficient multiplexed gene activation in plants.
Pan C, Wu X, Markel K, Malzahn AA, Kundagrami N, Sretenovic S, Zhang Y, Cheng Y, Shih PM, Qi Y
Nat Plants. 2021 Jul;7(7):942-953. doi: 10.1038/s41477-021-00953-7. Epub 2021 Jun 24. PubMed Article

Plasmids from Article

ID Plasmid Purpose
158393pYPQ131-tRNA2.0Golden Gate entry vector to express the 1st gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoter
158394pYPQ132-tRNA2.0Golden Gate entry vector to express the 2nd gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoter
158395pYPQ133-tRNA2.0Golden Gate entry vector to express the 3rd gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoter
158396pYPQ134-tRNA2.0Golden Gate entry vector to express the 4th gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoter
158397pYPQ135-tRNA2.0Golden Gate entry vector to express the 5th gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoter
158398pYPQ136-tRNA2.0Golden Gate entry vector to express the 6th gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoter
158400pYPQ143-ZmUbi-tRNAGateway entry clone and Golden Gate recipient for pYPQ131-tRNA2.0 to pYPQ133-tRNA2.0; assembly of 3 gRNAs
158402pYPQ144-ZmUbi-tRNAGateway entry clone and Golden Gate recipient for pYPQ131-tRNA2.0 to pYPQ134-tRNA2.0; assembly of 4 gRNAs
158403pYPQ145-ZmUbi-tRNAGateway entry clone and Golden Gate recipient for pYPQ131-tRNA2.0 to pYPQ135-tRNA2.0; assembly of 5 gRNAs
158404pYPQ146-ZmUbi-tRNAGateway entry clone and Golden Gate recipient for pYPQ131-tRNA2.0 to pYPQ136-tRNA2.0; assembly of 6 gRNAs
158406pYPQ141-ZmUbi-RZ-Aac.4Express single gRNA with Aac.4 scaffold (with four MS2 binding sites) under ZmUbi promoter
158407pYPQ141-ZmUbi-RZ-Aa3.8.5Express single gRNA with Aa3.8.5 scaffold (with four MS2 binding sites) under ZmUbi promoter
158408pYPQ-dpcoCas9-Act3.0CRISPR-Act3.0 system containing dpcoCas9-VP64 fusion protein, T2A linked MS2-SunTag fusion protein, and ScFv-sfGFP-2xTAD activator.
158409pYPQ-dpcoCas9-TVCRISPR-dpcoCas9 fused with TV activator for transcriptional activation
158410pYPQ-dpcoCas9-SunTagCRISPR-dpcoCas9 fused with SunTag recruiting VP64 activators for transcriptional activation
158411pYPQ-dpcoCas9-EV2.1CRISPR-dpcoCas9 fused with EDLL activator for transcriptional activation
158413pYPQ-dAaCas12b-Act3.0CRISPR-Act3.0 system containing dAaCas12b-VP64 fusion protein, T2A linked MS2-SunTag fusion protein, and ScFv-sfGFP-2xTAD activator.
158414pYPQ-dzCas9-Act3.0CRISPR-Act3.0 system containing dzCas9-VP64 fusion protein, T2A linked MS2-SunTag fusion protein, and ScFv-sfGFP-2xTAD activator.
158415pYPQ-dzCas9-NG-Act3.0CRISPR-Act3.0 system containing dzCas9-NG-VP64 fusion protein, T2A linked MS2-SunTag fusion protein, and ScFv-sfGFP-2xTAD activator.
158416pYPQ-dSpRY-Act3.0CRISPR-Act3.0 system containing dSpRYCas9-VP64 fusion protein, T2A linked MS2-SunTag fusion protein, and ScFv-sfGFP-2xTAD activator.
158578pYPQ142-ZmUbi-tRNAGateway entry clone and Golden Gate recipient for pYPQ131-tRNA2.0 and pYPQ132-tRNA2.0; assembly of 2 gRNAs
167158pYPQ134B2.0Golden Gate entry vector to express the 4th gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoter
167159pYPQ135B2.0Golden Gate entry vector to express the 5th gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoter
167160pYPQ136B2.0Golden Gate entry vector to express the 6th gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoter
167161pYPQ141D-gRNA2.1Express single gRNA with gRNA2.1 scaffold (with four MS2 binding sites) under OsU3 promoter

Antibodies from Article