Pharmacological enhancement of KCC2 gene expression exerts therapeutic effects on human Rett syndrome neurons and Mecp2 mutant mice.
Tang X, Drotar J, Li K, Clairmont CD, Brumm AS, Sullins AJ, Wu H, Liu XS, Wang J, Gray NS, Sur M, Jaenisch R
Sci Transl Med. 2019 Jul 31;11(503). pii: 11/503/eaau0164. doi: 10.1126/scitranslmed.aau0164. PubMed Article
Tang X, Drotar J, Li K, Clairmont CD, Brumm AS, Sullins AJ, Wu H, Liu XS, Wang J, Gray NS, Sur M, Jaenisch R
Sci Transl Med. 2019 Jul 31;11(503). pii: 11/503/eaau0164. doi: 10.1126/scitranslmed.aau0164. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 158576 | human SLC12A5-V5-HA-2A-Luciferase repair template | For targeted insertion of V5-HA-2A-Luciferase reporter before the stop codon of the human SLC12A5 gene that encodes neuronal chloride transporter KCC2 protein. |
| 158577 | sgRNA targeting human SLC12A5 gene stop codon | For generate double-strand DNA break near the stop codon of the human SLC12A5 gene that encodes neuronal chloride transporter KCC2 protein. |