Addgene: Acute disruption of the synaptic vesicle membrane protein synaptotagmin 1 using knockoff in mouse hippocampal neurons.

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Edwin Chapman
Vevea et al

Acute disruption of the synaptic vesicle membrane protein synaptotagmin 1 using knockoff in mouse hippocampal neurons.
Vevea JD, Chapman ER
Elife. 2020 Jun 9;9. pii: 56469. doi: 10.7554/eLife.56469. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
158763	pEF NS34a-msGFP-A'Q-mito	Expression of self-cleaving membrane targeting peptide containing the OMP25 C-terminal targeting sequence, a modified P6P4 NS5A/5B cleavage site, msGFP and the NS3/4A protease.
158764	pEF NS34a-msGFP-'Q-mito	Expression of self-cleaving membrane targeting peptide containing the OMP25 C-terminal targeting sequence, a modified P6P4 NS5A/5B cleavage site, msGFP and the NS3/4A protease.
158765	pEF NS34a-msGFP-'S-mito	Expression of self-cleaving membrane targeting peptide containing the OMP25 C-terminal targeting sequence, the P6P4 NS5A/5B cleavage site, msGFP and the NS3/4A protease.
158766	pF(UG) hSyn NS34a-msGFP-A'Q-mito	Lentiviral expression of self-cleaving membrane targeting peptide containing the OMP25 C-terminal targeting sequence, a modified P6P4 NS5A/5B cleavage site, msGFP and the NS3/4A protease.
158767	pF(UG) hSyn NS34a-msGFP-'Q-mito	Lentiviral expression of self-cleaving membrane targeting peptide containing the OMP25 C-terminal targeting sequence, a modified P6P4 NS5A/5B cleavage site, msGFP and the NS3/4A protease.
158768	pF(UG) hSyn NS34a-msGFP-'S-mito	Lentiviral expression of self-cleaving membrane targeting peptide containing the OMP25 C-terminal targeting sequence, the P6P4 NS5A/5B cleavage site, msGFP and the NS3/4A protease.
158769	pF(UG) CamKII sf iGluSnFR	iGluSnFR glutamate sensor containing a Golgi export sequence and and an ER exit motif for improved trafficking to the plasma membrane.
158770	pF(UG) hSyn SYP-SELF	Expression of synaptophysin 1 variant with self-cleaving c-terminal tail.
158771	pF(UG) hSyn Syt1-A'Q-4xGS-NS34a-FLAG (codopt)	Expression of synaptotagmin 1 variant with self-cleaving C2 domain.

Antibodies from Article