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Farnesylated and methylated KRAS4b: high yield production of protein suitable for biophysical studies of prenylated protein-lipid interactions.
Gillette WK, Esposito D, Abreu Blanco M, Alexander P, Bindu L, Bittner C, Chertov O, Frank PH, Grose C, Jones JE, Meng Z, Perkins S, Van Q, Ghirlando R, Fivash M, Nissley DV, McCormick F, Holderfield M, Stephen AG
Sci Rep. 2015 Nov 2;5:15916. doi: 10.1038/srep15916. PubMed Article

Plasmids from Article

ID Plasmid Purpose
159539R989-X02-566: His6-MBP-tev-G-Hs.KRAS4b(1-169)E. coli protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-169)
159540R989-X08-566: His6-MBP-tev-G-Hs.KRAS4b(1-169) G12CE. coli protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-169) G12C
159541R989-X09-566: His6-MBP-tev-G-Hs.KRAS4b(1-169) G12DE. coli protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-169) G12D
159542R989-X10-566: His6-MBP-tev-G-Hs.KRAS4b(1-169) G12VE. coli protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-169) G12V
159543R989-X11-566: His6-MBP-tev-G-Hs.KRAS4b(1-169) G13DE. coli protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-169) G13D
159544R989-X12-566: His6-MBP-tev-G-Hs.KRAS4b(1-169) Q61LE. coli protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-169) Q61L
159545R989-X13-566: His6-MBP-tev-G-Hs.KRAS4b(1-169) Q61RE. coli protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-169) Q61R
159546R989-X05-566: His6-MBP-tev-G-Hs.KRAS4b(1-188)E. coli protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-188)
159547R989-X27-566: His6-MBP-tev-G-Hs.KRAS4b(1-188) G12CE. coli protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-188) G12C
159548R989-X28-566: His6-MBP-tev-G-Hs.KRAS4b(1-188) G12DE. coli protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-188) G12D
159549R989-X29-566: His6-MBP-tev-G-Hs.KRAS4b(1-188) G12VE. coli protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-188) G12V
159550R989-X30-566: His6-MBP-tev-G-Hs.KRAS4b(1-188) G13DE. coli protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-188) G13D
159551R989-X31-566: His6-MBP-tev-G-Hs.KRAS4b(1-188) Q61LE. coli protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-188) Q61L
159552R989-X32-566: His6-MBP-tev-G-Hs.KRAS4b(1-188) Q61RE. coli protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-188) Q61R
159553R989-X05-636: His6-MBP-tev-G-Hs.KRAS4b(1-188)Baculovirus protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-188)
159554R989-X27-636: His6-MBP-tev-G-Hs.KRAS4b(1-188) G12CBaculovirus protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-188) G12C
159555R989-X28-636: His6-MBP-tev-G-Hs.KRAS4b(1-188) G12DBaculovirus protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-188) G12D
159556R989-X29-636: His6-MBP-tev-G-Hs.KRAS4b(1-188) G12VBaculovirus protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-188) G12V
159557R989-X30-636: His6-MBP-tev-G-Hs.KRAS4b(1-188) G13DBaculovirus protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-188) G13D
159558R989-X31-636: His6-MBP-tev-G-Hs.KRAS4b(1-188) Q61LBaculovirus protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-188) Q61L
159559R989-X32-636: His6-MBP-tev-G-Hs.KRAS4b(1-188) Q61RBaculovirus protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-188) Q61R
159560R701-X52-566: His6-MBP-tev-Avi-Hs.KRAS4b(2-169)E. coli protein expression of His6-MBP-tev-Avi-Hs.KRAS4b(2-169)
159561R701-X53-566: His6-MBP-tev-Avi-Hs.KRAS4b(2-169) G12CE. coli protein expression of His6-MBP-tev-Avi-Hs.KRAS4b(2-169) G12C
159562R701-X54-566: His6-MBP-tev-Avi-Hs.KRAS4b(2-169) G12DE. coli protein expression of His6-MBP-tev-Avi-Hs.KRAS4b(2-169) G12D
159563R701-X55-566: His6-MBP-tev-Avi-Hs.KRAS4b(2-169) G12VE. coli protein expression of His6-MBP-tev-Avi-Hs.KRAS4b(2-169) G12V
159564R701-X56-566: His6-MBP-tev-Avi-Hs.KRAS4b(2-169) G13DE. coli protein expression of His6-MBP-tev-Avi-Hs.KRAS4b(2-169) G13D
159565R701-X57-566: His6-MBP-tev-Avi-Hs.KRAS4b(2-169) Q61LE. coli protein expression of His6-MBP-tev-Avi-Hs.KRAS4b(2-169) Q61L
159566R701-X58-566: His6-MBP-tev-Avi-Hs.KRAS4b(2-169) Q61RE. coli protein expression of His6-MBP-tev-Avi-Hs.KRAS4b(2-169) Q61R
159567R701-X08-566: His6-MBP-tev-Avi-Hs.KRAS4b(2-188)E. coli protein expression of His6-MBP-tev-Avi-Hs.KRAS4b(2-188)
159568R701-X20-566: His6-MBP-tev-Avi-Hs.KRAS4b(2-188) G12CE. coli protein expression of His6-MBP-tev-Avi-Hs.KRAS4b(2-188) G12C
159569R701-X21-566: His6-MBP-tev-Avi-Hs.KRAS4b(2-188) G12DE. coli protein expression of His6-MBP-tev-Avi-Hs.KRAS4b(2-188) G12D
159570R701-X19-566: His6-MBP-tev-Avi-Hs.KRAS4b(2-188) G12VE. coli protein expression of His6-MBP-tev-Avi-Hs.KRAS4b(2-188) G12V
159571R701-X22-566: His6-MBP-tev-Avi-Hs.KRAS4b(2-188) G13DE. coli protein expression of His6-MBP-tev-Avi-Hs.KRAS4b(2-188) G13D
159572R701-X23-566: His6-MBP-tev-Avi-Hs.KRAS4b(2-188) Q61LE. coli protein expression of His6-MBP-tev-Avi-Hs.KRAS4b(2-188) Q61L
159573R701-X24-566: His6-MBP-tev-Avi-Hs.KRAS4b(2-188) Q61RE. coli protein expression of His6-MBP-tev-Avi-Hs.KRAS4b(2-188) Q61R
159574pDest-636Gateway Destination vector for protein expression in baculovirus

Antibodies from Article