NAADP-regulated two-pore channels drive phagocytosis through endo-lysosomal Ca(2+) nanodomains, calcineurin and dynamin.
Davis LC, Morgan AJ, Galione A
EMBO J. 2020 Jul 15;39(14):e104058. doi: 10.15252/embj.2019104058. Epub 2020 Jun 8. PubMed Article
Davis LC, Morgan AJ, Galione A
EMBO J. 2020 Jul 15;39(14):e104058. doi: 10.15252/embj.2019104058. Epub 2020 Jun 8. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 207141 | TPC2-G-GECO1.2 | Fusion of Ca2+ reporter and lysosomal Ca2+-permeable channel |
| 207142 | TPC2-D276K-G-GECO1.2 | Fusion of Ca2+ reporter and pore-dead lysosomal Ca2+-permeable channel |
| 207143 | TPC1-G-GECO1.2 | Fusion of Ca2+ reporter and endosomal Ca2+-permeable channel |
| 207144 | TRPML1-G-GECO1.2-ERES | Fusion of Ca2+ reporter and lysosomal Ca2+-permeable channel, with ER-export signal |
| 207145 | LAMP1-mCherry-FRB* | Lysosome-targeted rapalog dimerizer |
| 207146 | FKBP12-CB2x2-mTagBFP2-HAx2 | Tandem calcium-buffer with rapalog dimerizer |
| 207147 | FKBP12-CB2mutx2-mTagBFP2-HAx2 | Tandem mutant calcium-buffer with rapalog dimerizer |