Dual DNA and protein tagging of open chromatin unveils dynamics of epigenomic landscapes in leukemia.
Lee JD, Paulo JA, Posey RR, Mugoni V, Kong NR, Cheloni G, Lee YR, Slack FJ, Tenen DG, Clohessy JG, Gygi SP, Pandolfi PP
Nat Methods. 2021 Mar;18(3):293-302. doi: 10.1038/s41592-021-01077-8. Epub 2021 Mar 1. PubMed Article
Lee JD, Paulo JA, Posey RR, Mugoni V, Kong NR, Cheloni G, Lee YR, Slack FJ, Tenen DG, Clohessy JG, Gygi SP, Pandolfi PP
Nat Methods. 2021 Mar;18(3):293-302. doi: 10.1038/s41592-021-01077-8. Epub 2021 Mar 1. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 160081 | pTXB1-Tn5-F | Bacterial expression of C-terminally FLAG-tagged Tn5 transposase |
| 160083 | pTXB1-APEX2-F | Bacterial expression of C-terminally FLAG-tagged APEX2 peroxidase |
| 160084 | pTXB1-TP1 | Bacterial expression of Tn5-APEX2 fusion protein, no linker |
| 160085 | pTXB1-TP2 | Bacterial expression of Tn5-APEX2 fusion protein with linker PAPAP |
| 160086 | pTXB1-TP3 | Bacterial expression of Tn5-APEX2 fusion protein with linker AEAAAKEAAAKA |
| 160087 | pTXB1-TP4 | Bacterial expression of Tn5-APEX2 fusion protein with linker (GGGGS)4 |
| 160088 | pTXB1-TP5 | Bacterial expression of Tn5-APEX2 fusion protein with linker GSGAGA |