Combinatorial assembly of clone libraries using site-specific recombination.
Wall VE, Garvey LA, Mehalko JL, Procter LV, Esposito D
Methods Mol Biol. 2014;1116:193-208. doi: 10.1007/978-1-62703-764-8_14. PubMed Article
Wall VE, Garvey LA, Mehalko JL, Procter LV, Esposito D
Methods Mol Biol. 2014;1116:193-208. doi: 10.1007/978-1-62703-764-8_14. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 161878 | pDest-623 (attR4-attR3) | Multisite Gateway (attR4-attR3) vector for expression of multiple proteins using the Bac-to-Bac insect cell system |
| 161879 | pDest-624 (attR4-attR2) | Multisite Gateway (attR4-attR2) vector for expression of multiple proteins using the Bac-to-Bac insect cell system |
| 161880 | pDest-639 (attR4-attR2) | Multisite Gateway (attR4-attR2) vector for expression of multiple proteins using the Bac-to-Bac insect cell system |
| 161882 | pDest-658 (attR4-attR2) | Multisite Gateway (attR4-attR2) vector for lentivirus production |
| 161883 | pDest-659 (attR4-attR3) | Multisite Gateway (attR4-attR3) vector for lentivirus production |
| 161884 | pDest-663 (attR4-attR2) | Multisite Gateway (attR4-attR2) vector for lentivirus production |
| 161885 | pDest-665 (attR4-attR2) | Multisite Gateway (attR4-attR2) vector for lentivirus production |
| 161886 | pDest-667 (attR4-attR3) | Multisite Gateway (attR4-attR3) vector for lentivirus production |
| 161887 | pDest-669 (attR4-attR3) | Multisite Gateway (attR4-attR3) vector for lentivirus production |
| 161888 | pDest-676 (attR4-attR3) | Multisite Gateway (attR4-attR3) vector for lentivirus production |
| 161889 | pDest-686 (attR4-attR2) | Multisite Gateway (attR4-attR2) vector for lentivirus production |
| 161893 | pDest-894 (attR4-attR2) | Multisite Gateway (attR4-attR2) vector for expression of multiple proteins in E. coli |
| 161894 | pDest-302 (attR4-attR2) | Multisite Gateway (attR4-attR2) vector for mammalian protein expression |
| 161895 | pDest-305 (attR4-attR2) | Multisite Gateway (attR4-attR2) vector for mammalian protein expression |
| 161896 | pDest-311 (attR4-attR3) | Multisite Gateway (attR4-attR3) vector for mammalian protein expression |
| 161897 | pDest-312 (attR4-attR3) | Multisite Gateway (attR4-attR3) vector for mammalian protein expression |
| 161898 | pDest-313 (attR4-attR3) | Multisite Gateway (attR4-attR3) vector for mammalian protein expression |
| 162888 | C121-E01: rLuc | Standard Gateway entry clone encoding Renilla luciferase |
| 162889 | C121-E02: ffLuc2 | Standard Gateway entry clone encoding firefly luciferase |
| 162890 | C121-E06: gLuc | Standard Gateway entry clone encoding Gaussia luciferase |
| 162891 | C121-E12: NanoLuc | Standard Gateway entry clone encoding NanoLuc luciferase |
| 162892 | C122-E10: mCherry | Standard Gateway entry clone encoding mCherry fluorescent protein |
| 162893 | C122-E11: eGFP | Standard Gateway entry clone encoding eGFP fluorescent protein |
| 162894 | C122-E14: mKate | Standard Gateway entry clone encoding mKate fluorescent protein |
| 162895 | C122-E15: mOrange | Standard Gateway entry clone encoding mOrange fluorescent protein |
| 162896 | C122-E16: CyPet | Standard Gateway entry clone encoding CyPet fluorescent protein |
| 162897 | C122-E17: mPlum | Standard Gateway entry clone encoding mPlum fluorescent protein |
| 162898 | C122-E53: mClover3 | Standard Gateway entry clone encoding mClover fluorescent protein |
| 162899 | C122-E54: mRuby3 | Standard Gateway entry clone encoding mRuby3 fluorescent protein |
| 162900 | C125-E01: stuffer | Standard Gateway entry clone containing a non-transcribed/translated region for use in generating negative control vectors |
| 162901 | C231-E02: attB2r-NanoLuc-attB3 | Gateway attB2r/attB3 entry clone for construction of C-terminal NanoLuc fusion proteins |
| 162902 | C232-E05: attB2r-meGFP-attB3 | Gateway attB2r/attB3 entry clone for construction of C-terminal eGFP fusion proteins |
| 162903 | C232-E06: attB2r-mCherry-attB3 | Gateway attB2r/attB3 entry clone for construction of C-terminal mCherry fusion proteins |
| 162904 | C232-E08: attB2r-mTFP1-attB3 | Gateway attB2r/attB3 entry clone for construction of C-terminal mTFP1 fusion proteins |
| 162905 | C232-E25: attB2r-mVenus-attB3 | Gateway attB2r/attB3 entry clone for construction of C-terminal mVenus fusion proteins |
| 162906 | C232-E29: attB2r-mClover3-attB3 | Gateway attB2r/attB3 entry clone for construction of C-terminal mClover3 fusion proteins |
| 162907 | C232-E30: attB2r-mRuby3-attB3 | Gateway attB2r/attB3 entry clone for construction of C-terminal mRuby3 fusion proteins |
| 162908 | C232-E34: attB2r-iRFP670-attB3 | Gateway attB2r/attB3 entry clone for construction of C-terminal iRFP670 fusion proteins |
| 162909 | C234-E05: attB2r-V5-attB3 | Gateway attB2r/attB3 entry clone for construction of C-terminal V5 epitope tagged fusion proteins |
| 162910 | C234-E06: attB2r-FLAG-attB3 | Gateway attB2r/attB3 entry clone for construction of C-terminal FLAG epitope tagged fusion proteins |
| 162911 | C234-E07: attB2r-HA-attB3 | Gateway attB2r/attB3 entry clone for construction of C-terminal HA epitope tagged fusion proteins |
| 162912 | C234-E08: attB2r-3xFLAG-attB3 | Gateway attB2r/attB3 entry clone for construction of C-terminal triple-FLAG epitope tagged fusion proteins |
| 162913 | C234-E24: attB2r-Strep2-attB3 | Gateway attB2r/attB3 entry clone for construction of C-terminal Strep2 tagged fusion proteins |
| 162914 | C234-E25: attB2r-SNAP-attB3 | Gateway attB2r/attB3 entry clone for construction of C-terminal SNAP tagged fusion proteins |
| 162915 | C234-E26: attB2r-Halotag-attB3 | Gateway attB2r/attB3 entry clone for construction of C-terminal Halotag fusion proteins |
| 162916 | C238-E02: attB2r-BGHpolyA-attB3 | Gateway attB2r/attB3 entry clone containing a polyA sequence for transcription termination |
| 162917 | C238-E07: attB2r-SV40polyA-attB3 | Gateway attB2r/attB3 entry clone containing a polyA sequence for transcription termination |
| 162918 | C239-E02: attB2r-IRES-ffLuc2-eGFP-pA-attB3 | Gateway attB2r/attB3 entry clone for generation of polycistronic ffLuc2-eGFP reporter using an internal ribosome entry sequence (IRES) |
| 162919 | C239-E03: attB2r-IRES-ffLuc2-pA-attB3 | Gateway attB2r/attB3 entry clone for generation of polycistronic ffLuc2 reporter using an internal ribosome entry sequence (IRES) |
| 162920 | C413-E15: attB4-EF1p>-attB1r | Gateway attB4/attB1r entry clone for mammalian expression using the human EF1alpha promoter |
| 162921 | C413-E21: attB4-PGKp>-attB1r | Gateway attB4/attB1r entry clone for mammalian expression using the human PGK promoter |
| 162922 | C413-E22: attB4-polyhedrinp>-attB1r | Gateway attB4/attB1r entry clone for baculovirus expression using the AcMNPV polyhedrin promoter |
| 162923 | C413-E26: attB4-SV40p>-attB1r | Gateway attB4/attB1r entry clone for mammalian expression using the SV40 promoter |
| 162924 | C413-E31: attB4-TRE3Gp>-attB1r | Gateway attB4/attB1r entry clone for inducible mammalian expression using the TRE3G promoter |
| 162925 | C413-E33: attB4-UbCp>-attB1r | Gateway attB4/attB1r entry clone for mammalian expression using the human Ubiquitin C promoter |
| 162926 | C413-E34: attB4-mCMVp>-attB1r | Gateway attB4/attB1r entry clone for mammalian expression using the minimal CMV promoter |
| 162927 | C413-E36: attB4-CMV51p>-attB1r | Gateway attB4/attB1r entry clone for mammalian expression using an optimized human CMV promoter |
| 162928 | C413-E40: attB4-EFSp>-attB1r | Gateway attB4/attB1r entry clone for mammalian expression using the short EF1 promoter |
| 162929 | C413-E38: attB4-T7p>-attB1r | Gateway attB4/attB1r entry clone for bacterial expression using the bacteriophage T7 promoter |
| 162930 | C413-E45: attB4-hr5-ie1p>-attB1r | Gateway attB4/attB1r entry clone for transient insect expression using the ie1 promoter from AcMNPV |
| 162931 | C415-E01: attB4-stuffer-attB1r | Gateway attB4-attB1r entry clone containing a non-transcribed/translated region for use in generating negative control vectors |
| 162932 | C417-E01: attB4-polyhedrinp>His6-nostop-attB1r | Gateway attB4/attB1r entry clone for baculovirus expression of His6 tagged proteins using the polyhedrin promoter |
| 162933 | C417-E02: attB4-polyhedrinp>His6-MBP-nostop-attB1r | Gateway attB4/attB1r entry clone for baculovirus expression of His6-MBP tagged proteins using the polyhedrin promoter |
| 162934 | C417-E03: attB4-polyhedrinp>His6-GST-nostop-attB1r | Gateway attB4/attB1r entry clone for baculovirus expression of His6-GST tagged proteins using the polyhedrin promoter |
| 162935 | C417-E06: attB4-polyhedrinp>MBP-nostop-attB1r | Gateway attB4/attB1r entry clone for baculovirus expression of MBP tagged proteins using the polyhedrin promoter |
| 162936 | C417-E10: attB4-polyhedrinp>3xFLAG-nostop-attB1r | Gateway attB4/attB1r entry clone for baculovirus expression of 3xFLAG tagged proteins using the polyhedrin promoter |
| 162937 | C417-E12: attB4-polyhedrinp>Strep2x2-nostop-attB1r | Gateway attB4/attB1r entry clone for baculovirus expression of dual Strep2 tagged proteins using the polyhedrin promoter |
| 162938 | C511-E02: attB5r-NanoLuc-nostop-attB1r | Gateway attB5r/attB1r entry clone for generation of N-terminal Nanoluciferase fusion proteins |
| 162939 | C511-E15: attB5r-ffluc2-nostop-attB1r | Gateway attB5r/attB1r entry clone for generation of N-terminal Nanoluciferase fusion proteins |
| 162940 | C512-E01: attB5r-meGFP-nostop-attB1r | Gateway attB5r/attB1r entry clone for generation of N-terminal eGFP fusion proteins |
| 162941 | C512-E04: attB5r-mCherry-nostop-attB1r | Gateway attB5r/attB1r entry clone for generation of N-terminal mCherry fusion proteins |
| 162942 | C512-E11: attB5r-mTFP1-nostop-attB1r | Gateway attB5r/attB1r entry clone for generation of N-terminal mTFP1 fusion proteins |
| 162943 | C512-E15: attB5r-mVenus-nostop-attB1r | Gateway attB5r/attB1r entry clone for generation of N-terminal mVenus fusion proteins |
| 162944 | C512-E22: attB5r-mClover3-nostop-attB1r | Gateway attB5r/attB1r entry clone for generation of N-terminal mClover3 fusion proteins |
| 162945 | C512-E23: attB5r-mRuby3-nostop-attB1r | Gateway attB5r/attB1r entry clone for generation of N-terminal mRuby3 fusion proteins |
| 162946 | C512-E29: attB5r-mKate2-nostop-attB1r | Gateway attB5r/attB1r entry clone for generation of N-terminal mKate2 fusion proteins |
| 162947 | C512-E34: attB5r-iRFP670-nostop-attB1r | Gateway attB5r/attB1r entry clone for generation of N-terminal iRFP670 fusion proteins |
| 162948 | C513-E06: attB5r-pA-polyhedrinp>-attB1r | Gateway attB5r/attB1r entry clone containing an AcMNPV polyhedrin promoter with upstream polyA, for use in construction of polycistronic baculovirus expression vectors |
| 162949 | C513-E07: attB5r-<p10p-polyhedrinp>-attB1r | Gateway attB5r/attB1r entry clone containing divergent AcMNPV polyhedrin and p10 promoters, for use in construction of polycistronic baculovirus expression vectors |
| 162950 | C513-E08: attB5r-<p10p-polyhedrinp>H6-MBP-nostop-attB1r | Gateway attB5r/attB1r entry clone containing divergent AcMNPV polyhedrin and p10 promoters, for use in construction of polycistronic baculovirus expression vectors; allows His6-MBP fusion for the polyhedrin transcribed protein |
| 162951 | C514-E09: attB5r-RU5u-FLAG-nostop-attB1r | Gateway attB5r/attB1r entry clone for generation of N-terminal FLAG-tagged fusion proteins |
| 162952 | C514-E10: attB5r-RU5u-HA-nostop-attB1r | Gateway attB5r/attB1r entry clone for generation of N-terminal HA-tagged fusion proteins |
| 162953 | C514-E11: attB5r-RU5u-His6-nostop-attB1r | Gateway attB5r/attB1r entry clone for generation of N-terminal FLAG-tagged fusion proteins |
| 162954 | C514-E12: attB5r-RU5u-His6-eGFP-nostop-attB1r | Gateway attB5r/attB1r entry clone for generation of N-terminal eGFP-tagged fusion proteins |
| 162955 | C514-E13: attB5r-RU5u-His6-GST-nostop-attB1r | Gateway attB5r/attB1r entry clone for generation of N-terminal FLAG-tagged fusion proteins |
| 162956 | C514-E14: attB5r-RU5u-His6-Halotag-nostop-attB1r | Gateway attB5r/attB1r entry clone for generation of N-terminal Halotag-tagged fusion proteins |
| 162957 | C514-E15: attB5r-RU5u-His6-MBP-nostop-attB1r | Gateway attB5r/attB1r entry clone for generation of N-terminal FLAG-tagged fusion proteins |
| 162958 | C514-E23: attB5r-RU5u-3xFLAG-nostop-attB1r | Gateway attB5r/attB1r entry clone for generation of N-terminal 3xFLAG-tagged fusion proteins |
| 162959 | C514-E24: attB5r-RU5u-V5-nostop-attB1r | Gateway attB5r/attB1r entry clone for generation of N-terminal V5-tagged fusion proteins |
| 162960 | C514-E26: attB5r-RU5u-myc-nostop-attB1r | Gateway attB5r/attB1r entry clone for generation of N-terminal Myc-tagged fusion proteins |
| 162961 | C514-E42: attB5r-RU5u-Strep2-nostop-attB1r | Gateway attB5r/attB1r entry clone for generation of N-terminal Strep2-tagged fusion proteins |
| 162962 | C514-E44: attB5r-SNAP-nostop-attB1r | Gateway attB5r/attB1r entry clone for generation of N-terminal SNAP-tagged fusion proteins |
| 162963 | C514-E47: attB5r-Halotag-nostop-attB1r | Gateway attB5r/attB1r entry clone for generation of N-terminal Halotag-tagged fusion proteins |
| 162964 | C514-E51: attB5r-RBS-His6-MBP-nostop-attB1r | Gateway attB5r/attB1r entry clone for generation of N-terminal His6-MBP-tagged fusion proteins in bacterial expression |
| 162965 | C514-E54: attB5r-RBS-His6-Halotag-nostop-attB1r | Gateway attB5r/attB1r entry clone for generation of N-terminal His6-Halotag-tagged fusion proteins in bacterial expression |
| 162966 | C514-E59: attB5r-RU5u-2xHA-nostop-attB1r | Gateway attB5r/attB1r entry clone for generation of N-terminal dual HA-tagged fusion proteins |
| 162967 | C514-E67: attB5r-RU5u-Strep2x2-nostop-attB1r | Gateway attB5r/attB1r entry clone for generation of N-terminal dual Strep-tagged fusion proteins |
| 162968 | C515-E01: attB5r-stuffer-attB1r | Gateway attB5r-attB1r entry clone containing a non-transcribed/translated region for use in generating negative control vectors |
| 162969 | C413-E10: attB4-FerHp>-attB1r | Gateway attB4/attB1r entry clone for mammalian expression using the human EF1alpha promoter |
| 162970 | C413-E11: attB4-Grp78p>-attB1r | Gateway attB4/attB1r entry clone for mammalian expression using the human EF1alpha promoter |
| 162971 | C413-E19: attB4-mPol2p>-attB1r | Gateway attB4/attB1r entry clone for mammalian expression using the human EF1alpha promoter |
| 162972 | C453-E02: attB4-CAGp>-attB5 | Gateway attB4/attB5 entry clone for mammalian expression using the CAG promoter |
| 162973 | C453-E04: attB4-CMV51p>-attB5 | Gateway attB4/attB5 entry clone for mammalian expression using an optimized human CMV promoter |
| 162974 | C453-E09: attB4-Grp78p>-attB5 | Gateway attB4/attB5 entry clone for mammalian expression using the human Grp78 promoter |
| 162975 | C453-E14: attB4-EF1p>-attB5 | Gateway attB4/attB5 entry clone for mammalian expression using the human EF1alpha promoter |
| 162976 | C453-E17: attB4-PGKp>-attB5 | Gateway attB4/attB5 entry clone for mammalian expression using the human PGK promoter |
| 162977 | C453-E18: attB4-polyhedrinp>-attB5 | Gateway attB4/attB5 entry clone for baculovirus expression using the AcMNPV polyhedrin promoter |
| 162978 | C453-E21: attB4-FerHp>-attB5 | Gateway attB4/attB5 entry clone for mammalian expression using the human EF1alpha promoter |
| 162979 | C453-E23: attB4-TRE3Gp>-attB5 | Gateway attB4/attB5 entry clone for inducible mammalian expression using the TRE3G promoter |
| 162980 | C453-E24: attB4-UbCp>-attB5 | Gateway attB4/attB5 entry clone for mammalian expression using the human Ubiquitin C promoter |
| 162981 | C453-E25: attB4-mPol2p>-attB5 | Gateway attB4/attB5 entry clone for mammalian expression using the murine RNA polymerase 2 promoter |
| 162982 | C453-E26: attB4-T7p>-attB5 | Gateway attB4/attB1r entry clone for bacterial expression using the bacteriophage T7 promoter |
| 162983 | C455-E01: attB4-stuffer-attB5 | Gateway attB4-attB5 entry clone containing a non-transcribed/translated region for use in generating negative control vectors |