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The phospholipid PI(3,4)P2 is an apical identity determinant.
Roman-Fernandez A, Roignot J, Sandilands E, Nacke M, Mansour MA, McGarry L, Shanks E, Mostov KE, Bryant DM
Nat Commun. 2018 Nov 28;9(1):5041. doi: 10.1038/s41467-018-07464-8.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
161990GFP-2xPH-TAPP1 WTExpresses two tandem repeats of Tapp1 PH domain that binds to PI(3,4)P2. Fused to eGFP
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161991GFP-2xPH-TAPP1 R211L mutantExpresses two mutated tandem repeats of Tapp1 PH domain (phosphoinositide binding-deficient mutant of TAPP1, R211L, can't bind PI(3,4)P2). Fused to eGFP
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161992pQCXIH-tag RFPt- Rab11a WTRetroviral expression vector containing Rab11a tagged with RFP
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161993mCherry-C1 SNX9 RYK mutant R286A Y287A K288AMammalian expression vector containing a PIP-binding defective mutant of SNX9. Also contains mCherry tag
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161994mCherry-C1 SNX9 KR mutant K267N R327NMammalian expression vector containing a PIP-binding defective mutant of SNX9. Also contains mCherry tag
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161995pmCherry-C1-INPP4B WTMammalian expression vector containing INPP4B. Also contains mCherry tag
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161996pmCherry-C1-INPP4B C842A mutantMammalian expression vector containing a phosphatase dead mutant of INPP4B (C842A). Also contains mCherry tag
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161997pRevTRE SGFP2 Inpp5E WT shRNA resistantTet-responsive retroviral expression vector. Contains INPP5E. Also contains SGFP tag.
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161998INPP5D-eGFP WTMammalian expression vector, containing a GFP tagged version of INPP5D
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161999INPP5D-eGFP D673G mutantMammalian expression vector, containing a GFP tagged phosphatase dead version of INPP5D (D673G)
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162000pLEX303 GFP-PIK3C2ALentiviral expression vector containing PIK3C2A tagged with GFP
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162001pLEX303 GFP-PIK3C2BLentiviral expression vector containing PIK3C2B tagged with GFP
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162003pLKO.1 Hygro shRNA INPP4B KD2INPP4B shRNA
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162004pLKO.1 Puro shRNA INPP5E KD1INPP5E shRNA
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162005pLKO.1 Puro shRNA INPP5E KD2INPP5E shRNA
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162006pLKO.1 Puro shRNA PIK3C2A KD5PIK3C2alpha shRNA
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162008pLKO.1 Puro shRNA PIK3C2G KD1PIK3C2gamma shRNA
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162009pLKO.1 Puro shRNA PTEN KD2PTEN shRNA
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162010pLKO.1 Puro shRNA PTEN KD4PTEN shRNA
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162011pLKO.1 Puro shRNA ScrambleLentiviral negative control vector containing scramble shRNA
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162012pLKO.1 Puro shRNA SHIP1 KD1SHIP1 shRNA
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162013pLKO.1 Puro shRNA SHIP1 KD2SHIP1 shRNA
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162014pLKO.1 Puro shRNA SNX9 KD1SNX9 shRNA
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162015pLKO.1 Puro shRNA SNX9 KD2SNX9 shRNA
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Recombinant Antibodies from Article