A selectable, plasmid-based system to generate CRISPR/Cas9 gene edited and knock-in mosquito cell lines.
Rozen-Gagnon K, Yi S, Jacobson E, Novack S, Rice CM
Sci Rep. 2021 Jan 12;11(1):736. doi: 10.1038/s41598-020-80436-5.
(Link opens in a new window)
PubMed
(Link opens in a new window)
Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 162161 | pKRG3-mU6-PUb-3xFLAG-hSpCas9 | Expresses 3xFLAG tagged hSpCas9 (Ae. aegypti PUb promoter) with cloning site for sgRNAs (Ae. aegypti U6 promoter) |
| 162162 | pKRG3-mU6-PUb-hSpCas9 | Expresses hSpCas9 (Ae. aegypti PUb promoter) with cloning site for sgRNAs (Ae. aegypti U6 promoter) |
| 162163 | pKRG3-mU6-PUb-hSpCas9-pAc | Expresses hSpCas9-T2A-pAc (Ae. aegypti PUb promoter) with cloning site for sgRNAs (Ae. aegypti U6 promoter); puromycin selectable |
| 162164 | pSL1180-HR-Aag2-loxP-PUb-RFP-loxP-3xFLAG-AGO1 | Selectable homology donor template for Aag2 cell AGO1 |