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Binding and transport of SFPQ-RNA granules by KIF5A/KLC1 motors promotes axon survival.
Fukuda Y, Pazyra-Murphy MF, Silagi ES, Tasdemir-Yilmaz OE, Li Y, Rose L, Yeoh ZC, Vangos NE, Geffken EA, Seo HS, Adelmant G, Bird GH, Walensky LD, Marto JA, Dhe-Paganon S, Segal RA
J Cell Biol. 2021 Jan 4;220(1). pii: 211577. doi: 10.1083/jcb.202005051. PubMed Article

Plasmids from Article

ID Plasmid Purpose
166946UbC-Halo-SFPQ Lentiviral plasmid expressing Halo-tagged SFPQ protein from the UbC promoter
166947UbC-Halo-SFPQY527A Lentiviral plasmid expressing Halo-tagged SFPQ Y527A protein from the UbC promoter
166948pLV-EF1a-HaloSFPQ Lentiviral plasmid expressing Halo-tagged SFPQ protein from the EF1a promoter
166949pLV-EF1a-HaloSFPQY527A Lentiviral plasmid expressing Halo-tagged SFPQ Y527A protein from the EF1a promoter
166950pLV-EF1a-GFP-SFPQ-IRES-mCherry Lentiviral plasmid expressing GFP-tagged SFPQ protein with IRES-mCherry from the EF1a promoter
166951pLV-EF1a-GFP-SFPQY527A-IRES-mCherry Lentiviral plasmid expressing GFP-tagged SFPQ Y527A protein with IRES-mCherry from the EF1a promoter
166952pLV-EF1a-KIF5A-HA-IRES-Puro Lentiviral plasmid expressing HA-tagged KIF5A protein with IRES-Puro from the EF1a promoter
166953pLV-EF1a-KIF5AR280H-HA-IRES-Puro Lentiviral plasmid expressing HA-tagged KIF5A R280H protein with IRES-Puro from the EF1a promoter
166954FLAG-KIF5A Expresses FLAG-tagged KIF5A protein in pcDNA3.1
166955FLAG-KIF5A delta Tail Expresses FLAG-tagged KIF5A protein (1-820aa) in pcDNA3.1
166956FLAG-KIF5A tail only Expresses FLAG-tagged KIF5A protein (820-1027aa) in pcDNA3.1
166957FLAG-KIF5AR280H Expresses FLAG-tagged KIF5A R280H protein in pcDNA3.1
166958HA-KIF5A Expresses HA-tagged KIF5A protein in pcDNA3.1
166959HA-SFPQ Expresses HA-tagged SFPQ protein in pcDNA3.1
166960FLAG-SFPQ Expresses FLAG-tagged SFPQ protein in pcDNA3.1
166961FLAG-SFPQY527A Expresses FLAG-tagged SFPQ Y527A protein in pcDNA3.1
166962KLC1-MycExpresses FLAG-tagged SFPQ Y527A protein in pcDNA3.1
166963KLC1N343S-MycExpresses Myc-tagged KLC1 N343S protein in pcDNA3.1
166964Myc-KLC1 Expresses Myc-tagged KLC1 protein in pcDNA3.1
166965Myc-KLC1 delta Tail Expresses Myc-tagged KLC1 protein (1-495 aa) in pcDNA3.1

Antibodies from Article