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Allosteric Activation of SARS-CoV-2 RNA-Dependent RNA Polymerase by Remdesivir Triphosphate and Other Phosphorylated Nucleotides.
Wang B, Svetlov V, Wolf YI, Koonin EV, Nudler E, Artsimovitch I
mBio. 2021 Jun 29;12(3):e0142321. doi: 10.1128/mBio.01423-21. Epub 2021 Jun 22. PubMed Article

Plasmids from Article

ID Plasmid Purpose
166860pIA1362Expression of codon optimized SARS-COV-2 Nsp7 in E. coli
166861pIA1363Expression of codon optimized SARS-COV-2 Nsp8 in E. coli
166862pIA1385Codon optimized SARS-CoV-2 Nsp12 gene was cloned into a modified pRSFDuet-1 vector (Addgene #159107) for expression in E. coli.

Antibodies from Article