A highly multiplexed droplet digital PCR assay to measure the intact HIV-1 proviral reservoir.
Levy CN, Hughes SM, Roychoudhury P, Reeves DB, Amstuz C, Zhu H, Huang ML, Wei Y, Bull ME, Cassidy NAJ, McClure J, Frenkel LM, Stone M, Bakkour S, Wonderlich ER, Busch MP, Deeks SG, Schiffer JT, Coombs RW, Lehman DA, Jerome KR, Hladik F
Cell Rep Med. 2021 Apr 12;2(4):100243. doi: 10.1016/j.xcrm.2021.100243. eCollection 2021 Apr 20. PubMed Article
Levy CN, Hughes SM, Roychoudhury P, Reeves DB, Amstuz C, Zhu H, Huang ML, Wei Y, Bull ME, Cassidy NAJ, McClure J, Frenkel LM, Stone M, Bakkour S, Wonderlich ER, Busch MP, Deeks SG, Schiffer JT, Coombs RW, Lehman DA, Jerome KR, Hladik F
Cell Rep Med. 2021 Apr 12;2(4):100243. doi: 10.1016/j.xcrm.2021.100243. eCollection 2021 Apr 20. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 167347 | Seattle_IPDA_control_1_001 | ddPCR gating control for droplets containing all 5 targets (2 in pol) |
| 167348 | Seattle_IPDA_control_6_002 | ddPCR gating control for droplets containing either gag+5'pol targets OR 3'pol+tat targets |
| 167349 | Seattle_IPDA_control_9_003 | ddPCR gating control for droplets containing either gag+env targets OR tat + env targets |
| 167350 | Seattle_IPDA_control_14_004 | ddPCR gating control for droplets containing the env target |
| 167351 | Seattle_IPDA_control_17_005 | ddPCR gating control for droplets containing gat and tat targets |
| 167352 | Seattle_IPDA_control_29_006 | ddPCR gating control for droplets containing either env+5'pol targets OR env+3'pol targets |
| 167353 | Seattle_IPDA_control_32_007 | ddPCR gating control for droplets containing 5'pol or 3'pol targets |