An optimized CRISPR/Cas9 approach for precise genome editing in neurons.
Fang H, Bygrave AM, Roth RH, Johnson RC, Huganir RL
Elife. 2021 Mar 10;10. pii: 65202. doi: 10.7554/eLife.65202. PubMed Article
Fang H, Bygrave AM, Roth RH, Johnson RC, Huganir RL
Elife. 2021 Mar 10;10. pii: 65202. doi: 10.7554/eLife.65202. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 169416 | pSG | Used for guide cloning |
| 169417 | GluA1 (px330:2-guideCas9) | Expression of Cas9 and 2 guides |
| 169418 | pMiniT:SEP-GluA1 TKIT donor | Donor DNA for TKIT knockin of SEP to GluA1 in mouse |
| 169419 | pMiniT:myc-GluA1 TKIT donor | Donor DNA for TKIT knockin of Myc to GluA1 in mouse |
| 169420 | GluA2 (px330:2-guideCas9) | Expression of Cas9 and 2 guides |
| 169421 | pMiniT:SEP-GluA2 TKIT donor | Donor DNA for TKIT knockin of SEP to GluA2 in mouse |
| 169423 | pMiniT:myc-GluA2 TKIT donor | Donor DNA for TKIT knockin of Myc to GluA2 in mouse |
| 169424 | GluA3 (px330:2-guideCas9) | Expression of Cas9 and 2 guides |
| 169425 | pMiniT:SEP-GluA3 TKIT donor | Donor DNA for TKIT knockin of SEP to GluA3 in mouse |
| 169426 | GluN1 (px330:2-guideCas9) | Expression of Cas9 and 2 guides |
| 169427 | pMiniT:SEP-GluN1 TKIT donor | Donor DNA for TKIT knockin of SEP to NR1 in mouse |
| 169428 | GluN2A (px330:2-guideCas9) | Expression of Cas9 and 2 guides |
| 169429 | pMiniT:SEP-GluN2A TKIT donor | Donor DNA for TKIT knockin of SEP to NR2A in mouse |
| 169430 | GluN2B (px330:2-guideCas9) | Expression of Cas9 and 2 guides |
| 169431 | pMiniT:SEP-GluN2B TKIT donor | Donor DNA for TKIT knockin of SEP to NR2B in mouse |
| 169432 | Gephyrin (px330:2-guideCas9) | Expression of Cas9 and 2 guides |
| 169433 | pMiniT:GFP-Gephyrin TKIT donor | Donor DNA for TKIT knockin of GFP to Gephyrin in mouse |
| 169435 | beta2 (px330:2-guideCas9) | Expression of Cas9 and 2 guides |
| 169436 | pMiniT:SEP-beta2 TKIT donor | Donor DNA for TKIT knockin of SEP to Gabrb2 in mouse |
| 169437 | rGluA1 (px330:2-guideCas9) | Expression of Cas9 and 2 guides |
| 169438 | pHFS:SEP-rGluA1 TKIT donor | Donor DNA for TKIT knockin of SEP to GluA1 in rat |
| 169439 | rGluA2 (px330:2-guideCas9) | Expression of Cas9 and 2 guides |
| 169440 | pHFS:SEP-rGluA2 TKIT donor | Donor DNA for TKIT knockin of SEP to GluA2 in rat |
| 169441 | pAAV-SEP-GluA1 TKIT | Expression of 2 guides + donor DNA |
| 169442 | pAAV-SEP-GluA2 TKIT | Expression of 2 guides + donor DNA |
| 169443 | pX20 | Used for guide cloning with PCR |
| 169444 | pX52 | Used for guide cloning with PCR |