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High-performance microbial opsins for spatially and temporally precise perturbations of large neuronal networks.
Sridharan S, Gajowa MA, Ogando MB, Jagadisan UK, Abdeladim L, Sadahiro M, Bounds HA, Hendricks WD, Turney TS, Tayler I, Gopakumar K, Oldenburg IA, Brohawn SG, Adesnik H
Neuron. 2022 Jan 27. pii: S0896-6273(22)00008-3. doi: 10.1016/j.neuron.2022.01.008.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
170160pCAG-ChroME2f-mRuby2-STCation channelrhodopsin ChroME2f fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites
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170161pAAV-hSyn-DIO-ChroME-ST-P2A-H2B-mRuby3Cation channelrhodopsin ChroME targeted to the neuronal soma and proximal dendrites and separated from nuclear mRuby3 by P2A in a viral vector
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170163pAAV-CAG-DIO-ChroME2s-ST-P2A-H2B-mRuby3Cation channelrhodopsin ChroME2s targeted to the neuronal soma and proximal dendrites and separated from nuclear mRuby3 by P2A in a viral vector
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170175pAAV-TRE-ChroME2f-ST-P2A-NLS-mRuby3Cation channelrhodopsin ChroME2f targeted to the neuronal soma and proximal dendrites and separated from nuclear mRuby3 by P2A in a viral vector
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170177pAAV-TRE-ChroME2s-ST-P2A-NLS-mRuby3Cation channelrhodopsin ChroME2s targeted to the neuronal soma and proximal dendrites and separated from nuclear mRuby3 by P2A in a viral vector
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171150pAAV-CAG-DIO-ChroME2f-ST-P2A-H2B-mRuby3Cation channelrhodopsin ChroME2f targeted to the neuronal soma and proximal dendrites and separated from nuclear mRuby3 by P2A in a viral vector
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171154pCAG-mRuby3Encodes mRuby3 fluorophore
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171156pCAG-GCaMP6sEncodes GCaMP6s
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171158pCAG-ChroMD-mRuby2-STCation channelrhodopsin ChroMD fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites
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Recombinant Antibodies from Article