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Protein visualization and manipulation in Drosophila through the use of epitope tags recognized by nanobodies.
Xu J, Kim AR, Cheloha RW, Fischer FA, Li JSS, Feng Y, Stoneburner E, Binari R, Mohr SE, Zirin J, Ploegh HL, Perrimon N
Elife. 2022 Jan 25;11. pii: 74326. doi: 10.7554/eLife.74326. PubMed Article

Plasmids from Article

ID Plasmid Purpose
171564pMT-HGP-v3-Nb127D01-hIgGExpresses hIgG-tagged anti-CXCR2 (human) recombinant llama nanobody (Nb127D01-human IgG-TEV-Avi-His) in fly cells
171565pMT-HGP-v3-NbVHH05-hIgGExpresses hIgG-tagged anti-UBC6e (human) recombinant alpaca nanobody (NbVHH05-human IgG-TEV-Avi-His) in fly cells
171566pET-26b-Nb127D01-HA-HisBacterial expression of HA-His-tagged anti-CXCR2 (human) recombinant llama nanobody (Nb127D01-HA-His)
171567pET-26b-NbVHH05-HA-HisBacterial expression of HA-His-tagged anti-UBC6e (human) recombinant alpaca nanobody (NbVHH05-HA-His)
171568pET-26b-Nb127D01-ALFA-HisBacterial expression of ALFA-His-tagged anti-CXCR2 (human) recombinant llama nanobody (Nb127D01-ALFA-His)
171569pET-26b-NbVHH05-ALFA-HisBacterial expression of ALFA-His-tagged anti-UBC6e (human) recombinant alpaca nanobody (NbVHH05-ALFA-His)
171570pAW-NbVHH05-GFPExpresses GFP-tagged anti-UBC6e (human) recombinant alpaca nanobody (NbVHH05-GFP) in fly cells
171571pAW-Nb127D01-GFPExpresses GFP-tagged anti-CXCR2 (human) recombinant llama nanobody (Nb127D01-GFP) in fly cells
171572pAW-NbVHH05-mCherryExpresses mCherry-tagged anti-UBC6e (human) recombinant alpaca nanobody (NbVHH05-mCherry) in fly cells
171573pAW-Nb127D01-mCherryExpresses mCherry-tagged anti-CXCR2 (human) recombinant llama nanobody (Nb127D01-mCherry) in fly cells
171574pAW-BiP-NbVHH05-mCherry-KDELExpresses mCherry-tagged anti-UBC6e (human) recombinant alpaca nanobody (NbVHH05-mCherry) in fly cell ER membrane
171575pAW-BiP-Nb127D01-mCherry-KDELExpresses mCherry-tagged anti-CXCR2 (human) recombinant llama nanobody (Nb127D01-mCherry) in fly cell ER membrane
171576pAW-CD8-NbVHH05-GFPExpresses GFP-tagged anti-UBC6e (human) recombinant alpaca nanobody (NbVHH05-GFP) in fly cell cytomembrane
171577pAW-CD8-Nb127D01-GFPExpresses GFP-tagged anti-CXCR2 (human) recombinant llama nanobody (Nb127D01-GFP) in fly cell cytomembrane
171578pScarlessHD-C-3x127D01-DsRedFor scarless insertion of 3x127D01 NanoTag into C-term; Marked by the visible marker 3xP3-DsRed flanked by piggyBac termini.
171579pScarlessHD-N-3x127D01-DsRedFor scarless insertion of 3x127D01 NanoTag into N-term; Marked by the visible marker 3xP3-DsRed flanked by piggyBac termini.
171580pScarlessHD-C-3xVHH05-DsRedFor scarless insertion of 3xVHH05 NanoTag into C-term; Marked by the visible marker 3xP3-DsRed flanked by piggyBac termini.
171581pScarlessHD-N-3xVHH05-DsRedFor scarless insertion of 3xVHH05 NanoTag into N-term; Marked by the visible marker 3xP3-DsRed flanked by piggyBac termini.

Antibodies from Article