Programmed genome editing by a miniature CRISPR-Cas12f nuclease.
Wu Z, Zhang Y, Yu H, Pan D, Wang Y, Wang Y, Li F, Liu C, Nan H, Chen W, Ji Q
Nat Chem Biol. 2021 Sep 2. pii: 10.1038/s41589-021-00868-6. doi: 10.1038/s41589-021-00868-6. PubMed Article
Wu Z, Zhang Y, Yu H, Pan D, Wang Y, Wang Y, Li F, Liu C, Nan H, Chen W, Ji Q
Nat Chem Biol. 2021 Sep 2. pii: 10.1038/s41589-021-00868-6. doi: 10.1038/s41589-021-00868-6. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 171609 | p15a-AsCas12f-blaZ | AsCas12f1-based E. coli genome editing |
| 171610 | p15a-AsCas12f-apmR | AsCas12f1-based K. pneumoniae genome editing |
| 171611 | psgRNAv1-empty | sgRNA_v1 plasmid for AsCas12f1 in bacteria |
| 171612 | pET28a-SUMO-AsCas12f | AsCas12f1 heterologous expression plasmid |
| 171613 | pET28a-SUMO-AsCas12f-2NLS | NLS-AsCas12f1-NLS heterologous expression plasmid for RNP |
| 171614 | pCMV-AsCas12f1 | AsCas12f1-based Human cell genome editing |