Addgene: Fine-tuning the regulation of Cas9 expression levels for efficient CRISPR-Cas9 mediated recombination in Streptomyces.

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Eriko Takano
Ye et al

Fine-tuning the regulation of Cas9 expression levels for efficient CRISPR-Cas9 mediated recombination in Streptomyces.
Ye S, Enghiad B, Zhao H, Takano E
J Ind Microbiol Biotechnol. 2020 May;47(4-5):413-423. doi: 10.1007/s10295-020-02277-5. Epub 2020 May 4. PubMed Article

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ID	Plasmid	Purpose
172353	pCMU-4	CRISPR gene editing in Streptomyces, codon-optimized Cas9 behind theophylline riboswitch and D4 promoter, with custom gRNA

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