Fast, high-throughput production of improved rabies viral vectors for specific, efficient and versatile transsynaptic retrograde labeling.
Sumser A, Joesch M, Jonas P, Ben-Simon Y
Elife. 2022 Aug 30;11:e79848. doi: 10.7554/eLife.79848. PubMed Article
Sumser A, Joesch M, Jonas P, Ben-Simon Y
Elife. 2022 Aug 30;11:e79848. doi: 10.7554/eLife.79848. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 172359 | pAAV-DIO-Ef1a-TVA-2A-oG | Targeting envA-pseodotyped RVdG-CVS-N2c vectors for specific retrograde labeling specifically from cre-expressing neurons |
| 172360 | pAAV-DIO-Ef1a-TVA-2A-N2cG | Targeting envA-pseodotyped RVdG vectors for specific retrograde labeling specifically from cre-expressing neurons |
| 172361 | pAAV-FRT-EF1a-TVA-2A-N2cG | Targeting envA-pseodotyped RVdG-CVS-N2c vectors for specific retrograde labeling from Flp-expressing neurons |
| 172362 | pAAV-DIO-CaMKII-TVA-P2A-N2cG | Targeting envA-pseodotyped RVdG-CVS-N2c vectors for specific retrograde labeling from cre-expressing excitatory cortical neurons |
| 172363 | pAAV-SEO-CaMKII-TVA-P2A-N2cG | Targeting envA-pseodotyped RVdG-CVS-N2c vectors for specific retrograde labeling from excitatory cortical neurons which do not express cre-recombinase |
| 172364 | pAAV-mDLX-TVA-2A-N2cG | Targeting envA-pseodotyped RVdG-CVS-N2c vectors for specific retrograde labeling from inhibitory neurons |
| 172365 | pAAV-DIO-mDLX-TVA-2A-N2cG | Targeting envA-pseodotyped RVdG-CVS-N2c vectors for specific retrograde labeling from cre-expressing inhibitory neurons |
| 172366 | MMLV-CAG-TVA-IRES-Puro | Generation of TVA-expressing stable cell lines, resistant to the antibiotic Puromycin |
| 172367 | MMLV-CAG-SADB19_oG-IRES-Puro | Generation of stable cell lines expressing the rabies optimized glycoprotein (oG), resistant to the antibiotic Puromycin |
| 172368 | pLV-EF1a-N2c_envA-IRES-Neo | Generation of envA-expressing stable cell lines, resistant to the antibiotic Neomycin (Gentamicin/G418) |
| 172369 | MMLV-CAG-G_oT7pol-IRES-BSD | Generation of oT7pol-expressing stable cell lines, resistant to the antibiotic Blasticidin |
| 172370 | CVS-N2c-tdTomato-ChIEF | Production of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling. |
| 172371 | CVS-N2c-EGFP-ChIEF | Production of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling. |
| 172372 | CVS-N2c-EGFP-iCre | Production of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling. |
| 172373 | CVS-N2c-EGFP-FlpO | Production of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling. |
| 172374 | CVS-N2c-tdTomato-iCre | Production of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling. |
| 172375 | CVS-N2c-tdTomato-FlpO | Production of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling. |
| 172376 | CVS-N2c-mTurquoise | Production of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling. |
| 172377 | CVS-N2c-E2_Crimson | Production of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling. |
| 172378 | CVS-N2c-nl.mCherry-FlpO | Production of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling. |
| 172379 | CVS-N2c-nl.EGFP-FlpO | Production of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling. |
| 172380 | CVS-N2c-nl.EGFP-SypGFP | Production of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling. |
| 172381 | CVS-N2c-SypRFP | Production of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling. |
| 172382 | CVS-N2c-nl.EGFP-tdTomato | Production of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling. |
| 172383 | CVS-N2c-EYFP | Production of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling. |
| 172384 | CVS-N2c-mCitrine | Production of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling. |
| 172385 | CVS-N2c-nl.mCherry-GCaMP7s | Production of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling. |
| 172386 | CVS-N2c-nl.EGFP-jRGECO1a | Production of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling. |
| 172387 | CVS-N2c-jGCaMP8f | Production of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling. |
| 172388 | CVS-N2c-jGCaMP8m | Production of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling. |
| 172389 | CVS-N2c-jGCaMP8s | Production of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling. |