Programmable RNA targeting with the single-protein CRISPR effector Cas7-11.
Ozcan A, Krajeski R, Ioannidi E, Lee B, Gardner A, Makarova KS, Koonin EV, Abudayyeh OO, Gootenberg JS
Nature. 2021 Sep 6. pii: 10.1038/s41586-021-03886-5. doi: 10.1038/s41586-021-03886-5. PubMed Article
Ozcan A, Krajeski R, Ioannidi E, Lee B, Gardner A, Makarova KS, Koonin EV, Abudayyeh OO, Gootenberg JS
Nature. 2021 Sep 6. pii: 10.1038/s41586-021-03886-5. doi: 10.1038/s41586-021-03886-5. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 172500 | pDF0039 DisCas7-11 only expression with CRISPR array | Encodes the DisCas7-11 protein only for bacterial expression |
| 172501 | pDF0250 DisCas7-11 full locus with Golden gate acceptor site for spacer | Encodes the DisCas7-11 full locus along with a DR and golden gate site for cloning spacers |
| 172502 | pDF0044 CjcCas7-11 full locus with synthetic array | Encodes the CjcCas7-11 full locus for bacterial expression |
| 172503 | pDF0118 DisCas7-11 bacterial expression with Twinstrep-SUMO-Codon | Encodes the DisCas7-11 protein only for bacterial recombinant protein expression for purification |
| 172504 | pDF0163 GwCas7x3 bacterial expression with Twinstrep-SUMO-Codon | Encodes the GwCas7x3 protein only for bacterial recombinant protein expression for purification |
| 172505 | pDF0191 CjcCas7-11 bacterial expression with Twinstrep-SUMO-Codon | Encodes the CjcCas7-11 protein only for bacterial recombinant protein expression for purification |
| 172506 | pDF0229 DisCas7-11 bacterial expression vector with golden gate site for guides | Encodes DisCas7-11 protein alone with a golden gate site for spacer cloning |
| 172507 | pDF0159 pCMV - huDisCas7-11 mammalian expression | Encodes human codon optimized DisCas7-11 protein alone for expression in mammalian cells |
| 172508 | pDF0114 pU6-Eco31i-Eco31i-DisCas7-11 mature DR guide scaffold with golden gate site | Encodes the mature DisCas7-11 DR along with a golden gate site for spacer cloning |
| 172509 | pDF0234 pCMV - huDisCas7-11 dead mutant | Encodes human codon optimized catalytically dead DisCas7-11 protein alone for expression in mammalian cells |
| 172510 | pDF0237 pCMV - dead huDisCas7-11 - NES - huADAR2 | Encodes human codon optimized catalytically dead DisCas7-11-NES-huADAR2 fusion protein alone for expression in mammalian cells |
| 172511 | pDF0317 HvsCas7-11 mammalian expression | Encodes human codon optimized HvsCas7-11 protein alone for expression in mammalian cells |
| 172514 | pDF0338 pAAV U6-HvsCas7-11 DR Rev-BpiI golden gate backbone dual vector | Encodes HvsCas7-11 DR and golden gate site for spacer clonings in an AAV backbone with U6 promoter |
| 172515 | pDF0336 pAAV EFS-HvsCas7-11 for dual vector | Encodes HvsCas7-11 mammalian expression casette in AAV backbone |
| 172516 | pDF0352 HvsCas7-11 with C-term Nes mammalian expression | Encodes human codon optimized HvsCas7-11 protein with C-terminal NES tag for expression in mammalian cells |