Auxin-mediated protein depletion for metabolic engineering in terpene-producing yeast.
Lu Z, Peng B, Ebert BE, Dumsday G, Vickers CE
Nat Commun. 2021 Feb 16;12(1):1051. doi: 10.1038/s41467-021-21313-1. PubMed Article
Lu Z, Peng B, Ebert BE, Dumsday G, Vickers CE
Nat Commun. 2021 Feb 16;12(1):1051. doi: 10.1038/s41467-021-21313-1. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 165046 | pJAID6A | Integrative expression of yEGFP and rice auxin receptor gene OsTIR1. OsTIR1 is codon-optimised for expression in S. cerevisiae |
| 165047 | pJAID5Z | Integrative expression of AID* (mini auxin inducible degron)-yEGFP and rice auxin receptor gene OsTIR1. |
| 165048 | pJAID5VY | Integrative expression of CUP1-AID* (mini auxin inducible degron)-yEGFP and rice auxin receptor gene OsTIR1. |
| 165049 | pJAID6JY | Integrative expression of TRX1-AID* (mini auxin inducible degron)-yEGFP and rice auxin receptor gene OsTIR1. |
| 165050 | pJAID5WY | Integrative expression of SOD1-AID* (mini auxin inducible degron)-yEGFP and rice auxin receptor gene OsTIR1. |
| 165051 | pJAID5XY | Integrative expression of mRFP-CUP1-AID* (mini auxin inducible degron)-yEGFP and rice auxin receptor gene OsTIR1. |
| 165052 | pJAID9L | Integrative expression of yEGFP and rice auxin receptor gene OsTIR1. OsTIR1 is codon-optimised for expression in S. cerevisiae |
| 165053 | pJAID8Z | Integrative expression of yEGFP and SKP1-fusing rice auxin receptor gene OsTIR1. |
| 165054 | pJAID9L104 | Integrative expression of yEGFP-AID*-CUP1 and rice auxin receptor gene OsTIR1. |
| 165055 | pJAID9L105 | Integrative expression of nucleus-targeting yEGFP-AID*-CUP1 and rice auxin receptor gene OsTIR1. |
| 165056 | pJAID8Z104 | Integrative expression of yEGFP-AID*-CUP1 and SKP1-fusing rice auxin receptor gene OsTIR1. |
| 165057 | pJAID8Z105 | Integrative expression of nucleus-targeting yEGFP-AID*-CUP1 and SKP1-fusing rice auxin receptor gene OsTIR1. |
| 165058 | pILGFP5V1 | Integrative expression of CUP1-AID* (mini auxin inducible degron)-yEGFP |
| 165059 | pILGFP104 | Integrative expression of yEGFP-AID*(mini auxin inducible degron)-CUP1 |
| 165060 | pILGFP105 | Integrative expression of nucleus-targeting yEGFP-AID*(mini auxin inducible degron)-CUP1 |
| 165061 | pISKP1-TIR1 | Integrative expression of SKP1-fusing rice auxin receptor gene OsTIR1 |
| 165062 | pJT11F | Introducing GAL promoters-controlled monterpene limonene-producing module and Y-FAST and in S. cerevisiae |
| 165063 | pILGFP1D5 | Characterisation of yeast promoter using yEGFP as reporter gene and PGK1 terminator |
| 165064 | pILGFP1F5 | Characterisation of TEF1 promoter using yEGFP as reporter gene and PGK1 terminator |
| 165065 | pILGFP1H5 | Characterisation of TEF1 promoter using yEGFP as reporter gene and PGK1 terminator. |
| 165066 | pILGFPY2A | Characterisation of 2A-mediated bicistronic expression of Y-FAST and yEGFP |
| 165067 | pJT9RFR | Introducing GAL promoters-controlled sequiterpene nerolidol-producing module and Y-FAST and in S. cerevisiae |
| 165068 | pIR3DH8 | Knocking out GAL80 using KlURA3 marker |
| 165069 | pIR3DH8K | Knocking out GAL80 using KanMX4 marker |