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Histamine-induced biphasic activation of RhoA allows for persistent RhoA signaling.
Zhang JZ, Nguyen AH, Miyamoto S, Heller Brown J, McCulloch AD, Zhang J
PLoS Biol. 2020 Sep 3;18(9):e3000866. doi: 10.1371/journal.pbio.3000866. eCollection 2020 Sep. PubMed Article

Plasmids from Article

ID Plasmid Purpose
181996pcDNA3.1(+)-mCherry-p115Mammalian expression vector encoding p115 RhoGEF tagged with mCherry.
181997pcDNA3.1(+)-mCherry-p115(S240A)Mammalian expression vector encoding S240A-mutated p115 RhoGEF tagged with mCherry.
182061pcDNA3.1(+)-mTagBFP2-MRTF-AMammalian expression vector encoding myocardin-related transcription factor A tagged with mTagBFP2.
182062pcDNA3.1(+)-mTagBFP2-MRTF-BMammalian expression vector encoding myocardin-related transcription factor B tagged with mTagBFP2.

Antibodies from Article