A turquoise fluorescence lifetime-based biosensor for quantitative imaging of intracellular calcium.
van der Linden FH, Mahlandt EK, Arts JJG, Beumer J, Puschhof J, de Man SMA, Chertkova AO, Ponsioen B, Clevers H, van Buul JD, Postma M, Gadella TWJ Jr, Goedhart J
Nat Commun. 2021 Dec 9;12(1):7159. doi: 10.1038/s41467-021-27249-w. PubMed Article
van der Linden FH, Mahlandt EK, Arts JJG, Beumer J, Puschhof J, de Man SMA, Chertkova AO, Ponsioen B, Clevers H, van Buul JD, Postma M, Gadella TWJ Jr, Goedhart J
Nat Commun. 2021 Dec 9;12(1):7159. doi: 10.1038/s41467-021-27249-w. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 129626 | 3xnls-cpmTq2-Calcium-lifetime-sensor | Genetically encoded calcium sensor (GECI) that reports with lifetime and intensity changes |
| 129627 | Lck-cpmTq2-Calcium-lifetime-sensor | Genetically encoded calcium sensor (GECI) that reports with lifetime and intensity changes |
| 129628 | FHL-cpmTq2-Calcium-lifetime-sensor | Genetically encoded calcium sensor (GECI) that reports with lifetime and intensity changes |
| 145027 | pLV-H2B-Maroon-P2A-3xnls-Tq-Ca-FLITS | Lentiviral vector for a Turquoise calcium sensor (GECI) that reports with lifetime and intensity changes - co-expresses a nuclear marker |
| 145030 | pPB-3xnls-Tq-Ca-FLITS | piggyBac vector for expressing a Turquoise calcium sensor (GECI) that reports with lifetime and intensity changes |
| 191454 | N1-Tq-Ca-FLITS | Genetically encoded calcium sensor (GECI) Tq-Ca-FLITS that reports with lifetime and intensity changes |