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Visual detection of binary, ternary and quaternary protein interactions in fission yeast using a Pil1 co-tethering assay.
Yu ZQ, Liu XM, Zhao D, Xu DD, Du LL
J Cell Sci. 2021 Oct 1;134(19):jcs258774. doi: 10.1242/jcs.258774. Epub 2021 Oct 12. PubMed Article

Plasmids from Article

ID Plasmid Purpose
174012pDB4924pDUAL plasmid expressing Pil1-mCherry from 41nmt1 promoter
174013pDB1751pDUAL plasmid expressing GFP from 41nmt1 promoter
174014pDB4958pHIS3H plasmid expressing CFP-Atg6 from 41nmt1 promoter
174015pDB4961pHIS3H plasmid expressing 13Myc-Vps15 from 41nmt1 promoter
174016pDB4925pDUAL plasmid expressing Pil1-mCherry-Atg38(161-190) from 41nmt1 promoter
174017pDB4658pDUAL plasmid expressing GFP-Atg8 from 41nmt1 promoter
174018pDB4946pDUAL plasmid expressing Pil1-mCherry-Atg14 from 41nmt1 promoter
174019pDB4948pDUAL plasmid expressing Pil1-mCherry-Vps38 from 41nmt1 promoter
174020pDB4953pDUAL plasmid expressing GFP-Atg6 from 41nmt1 promoter

Antibodies from Article