Benjamin Kleinstiver Lab:
CRISPR-Cas9 vectors expressing mScarlet
Unpublished
Unpublished
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 174091 | pCMV-T7-SpCas9-BPNLS(SV40)-3xFLAG-P2A-mScarlet (MNW001) | CMV and T7 promoter expression plasmid for human codon optimized SpCas9 with a C-terminal bi-partite NLS, 3x flag tag, and P2A-mScarlet |
| 174136 | pCMV-T7-SpG-P2A-mScarlet (MNW006) | CMV and T7 promoter expression plasmid for human codon optimized SpG(D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-mScarlet |
| 174137 | pCMV-T7-SpRY-P2A-mScarlet (MNW002) | CMV and T7 promoter expression plasmid for human codon optimized SpRY(A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R); C-terminal bi-partite NLS, 3x flag tag, P2A-mScarlet |
| 174138 | pCMV-T7-SpCas9-VRER-P2A-mScarlet (MNW003) | CMV and T7 promoter expression plasmid for human codon optimized SpCas9-VRER(D1135V/G1218R/R1335E/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-mScarlet |
| 174139 | pCMV-T7-SpCas9-VRQR-P2A-mScarlet (MNW004) | CMV and T7 promoter expression plasmid for human codon optimized SpCas9-VRQR(D1135V/G1218R/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-mScarlet |
| 174140 | pCMV-T7-SpCas9-NG-P2A-mScarlet (MNW005) | CMV and T7 promoter expression plasmid for human codon optimized SpCas9-NG(L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-mScarlet |