Interrogation of the dynamic properties of higher-order heterochromatin using CRISPR-dCas9.
Gao Y, Han M, Shang S, Wang H, Qi LS
Mol Cell. 2021 Aug 13. pii: S1097-2765(21)00612-2. doi: 10.1016/j.molcel.2021.07.034. PubMed Article
Gao Y, Han M, Shang S, Wang H, Qi LS
Mol Cell. 2021 Aug 13. pii: S1097-2765(21)00612-2. doi: 10.1016/j.molcel.2021.07.034. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 174113 | pHR: pPGK PYL1-sfGFP-HP1Adel72 | Expression of PYL1-sfGFP-HP1Adel72 |
| 174114 | pHR: pPGK PYL1-sfGFP-HP1AI165E | Expression of PYL1-sfGFP-HP1AI165E |
| 174115 | pHR: pPGK mCherry-HP1A | Expression of mCherry-HP1A |
| 174116 | pHR: pPGK PYL1-sfGFP-NLS | Expression of PYL1-sfGFP-NLS |
| 174118 | pHR: pmU6 sgChr3q29 2xMS2 pUbC MCP-mCherry-p2a-Puro | Expression of sgRNA targeting Chr3q29 (chr3: 195478317 - 195506985, hg38) |
| 174119 | pHR: pmU6 sgChr13q34 2xMS2 pUbC MCP-mCherry-p2a-Puro | Expression of sgRNA targeting Chr13q34 (chr13: 112277011 - 112319170, hg38) |
| 174132 | pHR: pPGK PYL1-sfGFP-HP1A | Expression of PYL1-sfGFP-HP1A |