The AMBRA1 E3 ligase adaptor regulates the stability of cyclin D.
Chaikovsky AC, Li C, Jeng EE, Loebell S, Lee MC, Murray CW, Cheng R, Demeter J, Swaney DL, Chen SH, Newton BW, Johnson JR, Drainas AP, Shue YT, Seoane JA, Srinivasan P, He A, Yoshida A, Hipkins SQ, McCrea E, Poltorack CD, Krogan NJ, Diehl JA, Kong C, Jackson PK, Curtis C, Petrov DA, Bassik MC, Winslow MM, Sage J
Nature. 2021 Apr;592(7856):794-798. doi: 10.1038/s41586-021-03474-7. Epub 2021 Apr 14. PubMed Article
Chaikovsky AC, Li C, Jeng EE, Loebell S, Lee MC, Murray CW, Cheng R, Demeter J, Swaney DL, Chen SH, Newton BW, Johnson JR, Drainas AP, Shue YT, Seoane JA, Srinivasan P, He A, Yoshida A, Hipkins SQ, McCrea E, Poltorack CD, Krogan NJ, Diehl JA, Kong C, Jackson PK, Curtis C, Petrov DA, Bassik MC, Winslow MM, Sage J
Nature. 2021 Apr;592(7856):794-798. doi: 10.1038/s41586-021-03474-7. Epub 2021 Apr 14. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 174142 | pKHH030_sgCtrl#1 | Lentiviral vector expressing a control sgRNA that targets a safe harbor site |
| 174143 | pKHH030_sgCtrl#2 | Lentiviral vector expressing a control sgRNA that targets a safe harbor site |
| 174144 | pKHH030_sgRB1#1 | Lentiviral vector expressing a sgRNA against the human RB1 gene |
| 174145 | pKHH030_sgRB1#2 | Lentiviral vector expressing a sgRNA against the human RB1 gene |
| 174146 | pKHH030_sgAMBRA1#1 | Lentiviral vector expressing a sgRNA against the human AMBRA1 gene |
| 174147 | pKHH030_sgAMBRA1#2 | Lentiviral vector expressing a sgRNA against the human AMBRA1 gene |
| 174148 | lentiCRISPRv2_sgCtrl#1 | Lentiviral vector expressing Cas9 and a control sgRNA that targets a safe harbor site |
| 174149 | lentiCRISPRv2_sgCtrl#2 | Lentiviral vector expressing Cas9 and a control sgRNA that targets a safe harbor site |
| 174150 | lentiCRISPRv2_RB1#1 | Lentiviral vector expressing Cas9 and a sgRNA against the human RB1 gene |
| 174151 | lentiCRISPRv2_RB1#2 | Lentiviral vector expressing Cas9 and a sgRNA against the human RB1 gene |
| 174152 | lentiCRISPRv2_AMBRA1#1 | Lentiviral vector expressing Cas9 and a sgRNA against the human AMBRA1 gene |
| 174153 | lentiCRISPRv2_AMBRA1#2 | Lentiviral vector expressing Cas9 and a sgRNA against the human AMBRA1 gene |
| 174154 | pLV-hCCND1-HA | Lentiviral vector expressing HA-tagged human cyclin D1 |
| 174155 | pLV-hCCND1-T286A-HA | Lentiviral vector expressing HA-tagged human cyclin D1 with T286A mutation |
| 174156 | pLV-hCCND1-T2A-hCCND2-P2A-hCCND3 | Lentiviral vector expressing all three D-type cyclins (cyclin D1, cyclin D2, cyclin D3) from a single promoter |
| 174157 | pcDNA4-AMBRA1-3xFLAG | Vector for expression of 3xFLAG-tagged wild-type AMBRA1 |
| 174158 | pcDNA4-deltaH-AMBRA1-3xFLAG | Vector for expression of 3xFLAG-tagged truncated AMBRA1 that cannot bind DDB1 (aa 1-34 deleted) |
| 174159 | pPB-AMBRA1-3xFLAG | PiggyBac vector to stably express 3xFLAG-tagged AMBRA1 |
| 174160 | pPB-AMBRA1-501*-3xFLAG | PiggyBac vector to stably express 3xFLAG-tagged AMBRA1 truncated at amino acid 501 |
| 174161 | pPB-AMBRA1-217*-3xFLAG | PiggyBac vector to stably express 3xFLAG-tagged AMBRA1 truncated at amino acid 217 |