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A Single Nucleotide Change in the polC DNA Polymerase III in Clostridium thermocellum Is Sufficient To Create a Hypermutator Phenotype.
Lanahan A, Zakowicz K, Tian L, Olson DG, Lynd LR
Appl Environ Microbiol. 2022 Jan 11;88(1):e0153121. doi: 10.1128/AEM.01531-21. Epub 2021 Oct 20. PubMed Article

Plasmids from Article

ID Plasmid Purpose
174300pLT237Replicating plasmid with homology region for introducing PolCC669Y mutation. Confers thiamphenicol resistance.
174301pDGO186N-KS1Replicating plasmid with sgRNA cassette containing a killing spacer #1 targeting the wild type polC gene.
174302pDGO186N-KS2Replicating plasmid with sgRNA cassette containing a killing spacer #2 targeting the wild type polC gene.
174303pDGO186N-KS3Replicating plasmid with sgRNA cassette containing a killing spacer #3 targeting the wild type polC gene.

Antibodies from Article