NKD2 mediates stimulation-dependent ORAI1 trafficking to augment Ca(2+) entry in T cells.
Wu B, Woo JS, Vila P, Jew M, Leung J, Sun Z, Srikanth S, Gwack Y
Cell Rep. 2021 Aug 24;36(8):109603. doi: 10.1016/j.celrep.2021.109603. PubMed Article
Wu B, Woo JS, Vila P, Jew M, Leung J, Sun Z, Srikanth S, Gwack Y
Cell Rep. 2021 Aug 24;36(8):109603. doi: 10.1016/j.celrep.2021.109603. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 174323 | pLentiguide-puro_hNKD2sg#1 | gRNA targeting hNKD2 - encodes puromycin resistance |
| 174324 | pLentiguide-puro_hNKD2sg#2 | gRNA targeting hNKD2 - encodes puromycin resistance |
| 174325 | pLentiguide-puro_hNKD2sg#3 | gRNA targeting hNKD2 - encodes puromycin resistance |
| 174326 | pLentiguide-puro_hORAI1sg | gRNA targeting hORAI1 - encodes puromycin resistance |
| 174327 | MO70-C-FLAG-hNKD2 | encodes human NKD2 cDNA with a C-terminal FLAG tag in retroviral expression vector. Encodes GFP from IRES site and puromycin resistance |
| 174328 | pEGFPN1-hNKD2 | encodes human NKD2 cDNA with a C-terminal GFP-fusion. |
| 174329 | pcDNA3.1_hNKD2 | encodes human NKD2 cDNA with a C-terminal MycHis Tag. |
| 174330 | FG11F-C-FLAG-hNKD2 | encodes human NKD2 cDNA with a C-terminal FLAG tag in lentiviral vector. Does not encode any fluorescence/resistance marker. |
| 174331 | pCDNA3.1-NKD2S31A | encodes human NKD2 S31A mutant cDNA with a C-terminal MycHis Tag. |
| 174332 | pN1mcherry-NKD2S31A | encodes human NKD2 S31A mutant cDNA with a C-terminal mCherry fusion. |
| 174333 | pN1mcherry-NKD2EFMUT | encodes human NKD2 132DFD134>AFA mutant (two D mutated to A)cDNA with a C-terminal mCherry fusion. |
| 174334 | pN1mcherry-NKD2G2A | encodes human NKD2 G2A (2nd Gly residue to Ala) mutant cDNA with a C-terminal mCherry fusion. |
| 174335 | pEGFPC1-ORAI1-EC-HA | encodes hORAI1 with HA-tag in its extracellular loop 2 and an N-terminal GFP fusion (GFP-ORAI1-EC-HA). |
| 174336 | MO91-ORAI1N223A-TEV-pHluorin (ORAI1-EC-pHluorin) | The ORAI1N223A mutant containing-TEV-pHluorin-TEV sequence in the extracellular loop 2 |
| 176641 | pN1-mCherry-NKD2 | Expresses NKD2-mCherry fusion protein in mammalian cells |