Plexin-B2 orchestrates collective stem cell dynamics via actomyosin contractility, cytoskeletal tension and adhesion.
Junqueira Alves C, Dariolli R, Haydak J, Kang S, Hannah T, Wiener RJ, DeFronzo S, Tejero R, Gusella GL, Ramakrishnan A, Alves Dias R, Wojcinski A, Kesari S, Shen L, Sobie EA, Rodrigues Furtado de Mendonca JP, Azeloglu EU, Zou H, Friedel RH
Nat Commun. 2021 Oct 14;12(1):6019. doi: 10.1038/s41467-021-26296-7. PubMed Article
Junqueira Alves C, Dariolli R, Haydak J, Kang S, Hannah T, Wiener RJ, DeFronzo S, Tejero R, Gusella GL, Ramakrishnan A, Alves Dias R, Wojcinski A, Kesari S, Shen L, Sobie EA, Rodrigues Furtado de Mendonca JP, Azeloglu EU, Zou H, Friedel RH
Nat Commun. 2021 Oct 14;12(1):6019. doi: 10.1038/s41467-021-26296-7. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 98398 | pLKO-Tet-On-shRNA-Control | Lentivirus for expression of non-targeting control shRNA (Dox-inducible) |
| 98399 | pLKO-Tet-On-PLXNB2-shRNA1 | Lentivirus for expression of shRNA1 against human PLEXIN-B2 (Dox-inducible) |
| 98400 | pLKO-Tet-On-PLXNB2-shRNA2 | Lentivirus for expression of shRNA2 against human PLEXIN-B2 (Dox-inducible) |
| 156166 | pLenti-PGK-RAP1A-WT-puro | Lentiviral vector for expression of RAP1A-WT, with puromycin selection |
| 156167 | pLenti-PGK-RAP1A-E63-puro | Lentiviral vector for expression of RAP1A-E63, with puromycin selection |
| 156168 | pLenti-PGK-flag-RAP1B-V12-puro | Lentiviral vector for expression of flag-RAP1B-V12, with puromycin selection |
| 156169 | pLenti-PGK-flag-RAP1B-N17-puro | Lentiviral vector for expression of flag-RAP1B-N17, with puromycin selection |
| 156170 | pLenti-PGK-RAP2A-WT-puro | Lentiviral vector for expression of RAP2A-WT, with puromycin selection |
| 156171 | pLenti-PGK-RAP2A-V12-puro | Lentiviral vector for expression of RAP2A-V12, with puromycin selection |
| 156172 | pLenti-PGK-RAP2A-N17-puro | Lentiviral vector for expression of RAP2A-N17, with puromycin selection |
| 156173 | pLenti-PGK-RAP1A-WT-neo | Lentiviral vector for expression of RAP1A-WT, with neomycin section |
| 156174 | pLenti-PGK-RAP1A-E63-neo | Lentiviral vector for expression of RAP1A-E63, with neomycin selection |
| 156175 | pLenti-PGK-flag-RAP1B-V12-neo | Lentiviral vector for expression of flag-RAP1B-V12, with neomycin selection |
| 156176 | pLenti-PGK-flag-RAP1B-N17-neo | Lentiviral vector for expression of flag-RAP1B-N17, with neomycin selection |
| 156177 | pLenti-PGK-RAP2A-WT-neo | Lentiviral vector for expression of RAP2A-WT, with neomycin selection |
| 156178 | pLenti-PGK-RAP2A-V12-neo | Lentiviral vector for expression of RAP2A-V12, with neomycin selection |
| 156179 | pLenti-PGK-RAP2A-N17-neo | Lentiviral vector for expression of RAP2A-N17, with neomycin selection |
| 176846 | pLKO-Tet-On-YAP1-shRNA1 | lentiviral vector for doxycycline-inducible human YAP1 knockdown |
| 176847 | pLKO-Tet-On-YAP1-shRNA2 | lentiviral vector for doxycycline-inducible human YAP1 knockdown |
| 176848 | pLV-hPLXNB2_PGK_Neo | lentiviral vector for constitutive PLXNB2 overexpression |
| 176849 | pLV-CMVtight-PLXNB2 iOE | lentiviral vector for doxycycline-inducible PLXNB2 overexpression |
| 176850 | pLV-TRE3G-hYAP1-CMV-mCherry-T2A-Puro | lentiviral vector for doxycycline-inducible YAP1 overexpression |
| 176851 | pLV-TRE3G-YAP1-5SA-CMV-mCherry-T2A-Puro | lentiviral vector for doxycycline-inducible YAP1 5SA constitutively active expression |