Joseph Brzezinski Lab:
Brzezinski Dual Guide Donor/Activator/Repressor plasmids
Unpublished
Please see Addgene's curated Brzezinski Lab CRISPR Collection page for additional information about this set of plasmids.
Unpublished
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 175570 | pX458-Dual-Guide-Donor-Cas9-H2B-mCherry | Donor shuttle plasmid to enable ligation of a second U6-GuideRNA casssette into any pX458 family backbone via digestion of both plasmids with XbaI and KpnI. |
| 175571 | pX458-Ef1a-dCas9-VPR-H2B-GFP (CRISPRa) | Inactivated Cas9 (dCas9) fusion to VPR for the purpose of targeted activation |
| 175572 | pX458-Ef1a-dCas9-VPR-H2B-mCherry (CRISPRa) | Inactivated Cas9 (dCas9) fusion to VPR for the purpose of targeted activation |
| 175573 | pX458-Ef1a-dCas9-KRAB-MECP2-H2B-GFP (CRISPRi) | Inactivated Cas9 (dCas9) fusion to KRAB and MeCP2 for the purpose of targeted repression |
| 175574 | pX458-Ef1a-dCas9-KRAB-MECP2-H2B-mCherry (CRISPRi) | Inactivated Cas9 (dCas9) fusion to KRAB and MeCP2 for the purpose of targeted repression |