Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

Addgene
An Inactive Dispersin B Probe for Monitoring PNAG Production in Biofilm Formation.
Eddenden A, Kitova EN, Klassen JS, Nitz M
ACS Chem Biol. 2020 May 15;15(5):1204-1211. doi: 10.1021/acschembio.9b00907. Epub 2020 Jan 21. PubMed Article

Plasmids from Article

ID Plasmid Purpose
175783pET29b-eGFP-dspB(E184Q)-6xHisPlasmid for overexpression of recombinant His-tagged fusion protein eGFP-DspB(E184Q), used as a fluorescent probe for detection of biofilm polysaccharide PNAG.
175784pET29b-AviTag-eGFP-dspB(E184Q)-6xHisPlasmid for overexpression of recombinant AviTagged, His-tagged fusion protein eGFP-DspB(E184Q), used as a fluorescent probe for detection of biofilm polysaccharide PNAG.
175788pET29b-AviTag(K10A)-eGFP-dspB(E184Q)-6xHisPlasmid for overexpression of recombinant AviTagged(nonfunctional), His-tagged fusion protein eGFP-DspB(E184Q), used as a fluorescent probe for detection of biofilm polysaccharide PNAG.
175801pET29b-AviTag-eGFP-dspB(E184Q W330Y)-6xHisPlasmid for overexpression of recombinant AviTagged, His-tagged fusion protein eGFP-DspB(E184Q W330Y), used as a non-binding control for detection of biofilm polysaccharide PNAG.
175802pET29b-dspB(E184Q)-6xHisPlasmid for overexpression of recombinant His-tagged protein DspB(E184Q), used as a probe for binding to biofilm polysaccharide PNAG.

Antibodies from Article