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A synthetic gene circuit for imaging-free detection of signaling pulses.
Ravindran PT, McFann S, Thornton RH, Toettcher JE
Cell Syst. 2022 Feb 16;13(2):131-142.e13. doi: 10.1016/j.cels.2021.10.002. Epub 2021 Nov 4. PubMed Article

Plasmids from Article

ID Plasmid Purpose
176530pHR pUAS-dGFP pPGK-iRFPLentiviral vector expressing a UAS-driven destabilized GFP, as well as a PGK promoter-driven iRFP marker.
176531pPBJ pFOS-KTR-Gal4-PEST-tub3’ pCMV-TagBFPPiggyBAC vector expressing a FOS promoter driven "KGV" synthetic transcription factor and a CMV promoter driven BFP marker.

Antibodies from Article