The widespread IS200/IS605 transposon family encodes diverse programmable RNA-guided endonucleases.
Altae-Tran H, Kannan S, Demircioglu FE, Oshiro R, Nety SP, McKay LJ, Dlakic M, Inskeep WP, Makarova KS, Macrae RK, Koonin EV, Zhang F
Science. 2021 Oct;374(6563):57-65. doi: 10.1126/science.abj6856. Epub 2021 Sep 9. PubMed Article
Altae-Tran H, Kannan S, Demircioglu FE, Oshiro R, Nety SP, McKay LJ, Dlakic M, Inskeep WP, Makarova KS, Macrae RK, Koonin EV, Zhang F
Science. 2021 Oct;374(6563):57-65. doi: 10.1126/science.abj6856. Epub 2021 Sep 9. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 176534 | pHS0570 pET45b(+) T7/LacO His14-MBP-bdSUMO-CRISPR associated IscB-TwinStrep | Bacterial expression of His14-MBP-bdSUMO-CRISPR associated IscB-TwinStrep |
| 176535 | pHS0578 CRISPR associated IscB non-coding locus in pColA-Duet | Non-protein coding locus components from CRISPR-associated IscB |
| 176536 | pHS0663 pET45b(+) KraIscB-1 TwinStrep | Bacterial expression of KraIscB-1-TwinStrep |
| 176537 | pHS0667 KraIscB-1 non-coding locus in pCOLADuet-1 | Non-protein coding locus components from KraIscB-1 |
| 176538 | pHS0781 pcDNA3.1(+) CMV-SV40 NLS-AwaIscB-nucleoplasmin NLS-3xHA | Mammalian expression of nuclear localized AwaIscB |
| 176539 | pHS0806 His14-bdSUMO-AwaIscB | Bacterial expression of His14-bdSUMO-AwaIscB |
| 176540 | pHS0810 pcDNA3.1(+) CMV-SV40 NLS-OgeuIscB-nucleoplasmin NLS-3xHA | Mammalian expression of nuclear localized OgeuIscB |
| 176541 | pHS0812 hU6-BpiI-OgeuIscB omegaRNA scaffold | human U6-driven expression of OgeuIscB omegaRNA scaffold with BpiI site for guide cloning |
| 176542 | pHS0766 pET45b(+) His14-MBP-TEV-AloTnpB-2 3' extension | Bacterial expression of His14-MBP-TEV-AloTnpB-2 with 3' extension derived from locus |
| 176587 | pHS0923 pET45b(+) His14-MBP-TEV-AmaTnpB | Bacterial expression of His14-MBP-TEV-AmaTnpB |
| 176588 | pHS0537 CRISPR-associated IscB locus (Delaware Bay) in pBR322 with Fn spacer | Endogenous locus of CRISPR-associated IscB (Delaware Bay) with Fn spacer |
| 176589 | pHS0535 CRISPR-associated IscB (Chesapeake Bay) in pBR322 with Fn spacer | Endogenous locus of CRISPR-associated IscB (Chesapeake Bay) with Fn spacer |