Restriction of SARS-CoV-2 replication by targeting programmed -1 ribosomal frameshifting.
Sun Y, Abriola L, Niederer RO, Pedersen SF, Alfajaro MM, Silva Monteiro V, Wilen CB, Ho YC, Gilbert WV, Surovtseva YV, Lindenbach BD, Guo JU
Proc Natl Acad Sci U S A. 2021 Jun 29;118(26). pii: 2023051118. doi: 10.1073/pnas.2023051118. PubMed Article
Sun Y, Abriola L, Niederer RO, Pedersen SF, Alfajaro MM, Silva Monteiro V, Wilen CB, Ho YC, Gilbert WV, Surovtseva YV, Lindenbach BD, Guo JU
Proc Natl Acad Sci U S A. 2021 Jun 29;118(26). pii: 2023051118. doi: 10.1073/pnas.2023051118. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 177614 | pFRLuc-CoV2-WT | Dual luciferase reporter for SARS-CoV-2 programmed -1 ribosomal frameshifting |
| 177615 | pFRLuc-CoV2-delSS | Dual luciferase reporter for SARS-CoV-2 programmed -1 ribosomal frameshifting. Slippery sequence (SS) deleted (negative control). |
| 177616 | pFRLuc-CoV2-delStem1 | Dual luciferase reporter for SARS-CoV-2 programmed -1 ribosomal frameshifting. 3-nucleotide deletion in Stem 1 (negative control). |
| 177617 | pFRLuc-CoV2-FLucStop | Dual luciferase reporter for SARS-CoV-2 programmed -1 ribosomal frameshifting. In-frame stop codon inserted after FLuc (negative control). |
| 177618 | pCherryGFP-CoV2-WT | Dual fluorescent protein reporter for SARS-CoV-2 programmed -1 ribosomal frameshifting |
| 177619 | pCherryGFP-Inframe-noFSE | Dual fluorescent protein reporter for SARS-CoV-2 programmed -1 ribosomal frameshifting. No FSE was inserted. mCherry and GFP are expressed equally (positive control). |
| 177620 | pCherryGFP-CoV2-Inframe-stop | Dual fluorescent protein reporter for SARS-CoV-2 programmed -1 ribosomal frameshifting. GFP is in-frame with mCherry. Thus GFP translation is prevented by stop codon within FSE (negative control). |