Reconstitution of monoterpene indole alkaloid biosynthesis in genome engineered Nicotiana benthamiana.
Dudley QM, Jo S, Guerrero DAS, Chhetry M, Smedley MA, Harwood WA, Sherden NH, O'Connor SE, Caputi L, Patron NJ
Commun Biol. 2022 Sep 10;5(1):949. doi: 10.1038/s42003-022-03904-w. PubMed Article
Dudley QM, Jo S, Guerrero DAS, Chhetry M, Smedley MA, Harwood WA, Sherden NH, O'Connor SE, Caputi L, Patron NJ
Commun Biol. 2022 Sep 10;5(1):949. doi: 10.1038/s42003-022-03904-w. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 177019 | pEPQD0CM0065 | Level 0 CDS (with stop codon) part for MoClo assembly, AATG-GCTT |
| 177020 | pEPQD0CM0818 | Level 0 CDS (with stop codon) part for MoClo assembly, AATG-GCTT |
| 177021 | pEPQD0CM0063 | Level 0 CDS (with stop codon) part for MoClo assembly, AATG-GCTT |
| 177022 | pEPQD0CM0058 | Level 0 CDS (with stop codon) part for MoClo assembly, AATG-GCTT |
| 177023 | pEPQD0CM0059 | Level 0 CDS (with stop codon) part for MoClo assembly, AATG-GCTT |
| 177024 | pEPQD0CM0060 | Level 0 CDS (with stop codon) part for MoClo assembly, AATG-GCTT |
| 177025 | pEPQD0CM0068 | Level 0 CDS (with stop codon) part for MoClo assembly, AATG-GCTT |
| 177026 | pEPQD0CM0061 | Level 0 CDS (with stop codon) part for MoClo assembly, AATG-GCTT |
| 177027 | pEPQD0CM0062 | Level 0 CDS (with stop codon) part for MoClo assembly, AATG-GCTT |
| 177028 | pEPQD0CM0762 | Level 0 CDS (with stop codon) part for MoClo assembly, AATG-GCTT |
| 177029 | pEPQD0CM0763 | Level 0 CDS (with stop codon) part for MoClo assembly, AATG-GCTT |
| 177030 | pEPQD0CM0764 | Level 0 CDS (with stop codon) part for MoClo assembly, AATG-GCTT |
| 177031 | pEPQD0CM0765 | Level 0 CDS (with stop codon) part for MoClo assembly, AATG-GCTT |
| 177032 | pEPQD0CM0766 | Level 0 CDS (with stop codon) part for MoClo assembly, AATG-GCTT |
| 177033 | pEPQD0CM0910 | Level 0 CDS (with stop codon) part for MoClo assembly, AATG-GCTT |
| 177034 | pEPQD0CM0812 | Level 0 N-terminal tag part for MoClo assembly, CCAT-AATG |
| 177035 | pEPQD0CM0705 | Level 0 CDS (with stop codon) part for MoClo assembly, AATG-GCTT |
| 177036 | pUAP12008 | Level 0 promoter and 5'UTR part for MoClo assembly, GGAG-CCAT |
| 177037 | pEPKK0CM0480 | Level 0 promoter binding site part for MoClo assembly, nonstandard overlaps, CCAT-TACT |
| 177038 | pEPQD1CB0104 | MoClo Level 1, position 6, transcriptional unit for transient expression of P19 suppressor of gene silencing from Tomato Bushy Stunt Virus driven by 35S promoter |
| 177039 | pEPQD1CB0817 | MoClo Level 1, position 1, transcriptional unit for transient expression of truncated 3-hydroxy-3-methylglutaryl-coenzyme A reductase (AstHMGR1) from Avena strigosa driven by 35S promoter |
| 177040 | pEPQD1CB0107 | MoClo Level 1, position 1, transcriptional unit for transient expression of 1-deoxy-D-xylulose 5-phosphate synthase (CrDXS2) from Catharanthus roseus driven by 35S promoter; contains a chloroplast transit peptide |
| 177041 | pEPQD1CB0108 | MoClo Level 1, position 2, transcriptional unit for transient expression of geranyl pyrophosphate synthase (PaGPPS1) from Picea abies driven by 35S promoter |
| 177042 | pEPQD1CB0109 | MoClo Level 1, position 2, transcriptional unit for transient expression of geranyl pyrophosphate synthase (PaGPPS1) from Picea abies driven by 35S promoter; contains a chloroplast transit peptide |
| 177043 | pEPQD1CB0110 | MoClo Level 1, position 3, transcriptional unit for transient expression of geraniol synthase (CrGES) from Catharanthus roseus driven by 35S promoter |
| 177044 | pEPQD1CB0112 | MoClo Level 1, position 3, transcriptional unit for transient expression of geraniol synthase (CrGES) from Catharanthus roseus driven by 35S promoter; contains a chloroplast transit peptide |
| 177045 | pEPQD1CB0113 | MoClo Level 1, position 4, transcriptional unit for transient expression of geraniol 8-oxidase (CrG8H) from Catharanthus roseus driven by 35S promoter |
| 177046 | pEPQD1CB0114 | MoClo Level 1, position 4, transcriptional unit for transient expression of geraniol 8-oxidase (CrG8H) from Catharanthus roseus driven by 35S promoter; contains a chloroplast transit peptide |
| 177047 | pEPQD1CB0115 | MoClo Level 1, position 5, transcriptional unit for transient expression of 8-hydroxygeraniol oxidoreductase (CrGOR) from Catharanthus roseus driven by 35S promoter |
| 177048 | pEPQD1CB0116 | MoClo Level 1, position 5, transcriptional unit for transient expression of 8-hydroxygeraniol oxidoreductase (CrGOR) from Catharanthus roseus driven by 35S promoter; contains a chloroplast transit peptide |
| 177049 | pEPQD1CB0117 | MoClo Level 1, position 6, transcriptional unit for transient expression of iridoid synthase (CrISY) from Catharanthus roseus driven by 35S promoter |
| 177050 | pEPQD1CB0118 | MoClo Level 1, position 6, transcriptional unit for transient expression of iridoid synthase (CrISY) from Catharanthus roseus driven by 35S promoter; contains a chloroplast transit peptide |
| 177051 | pEPQD1CB0119 | MoClo Level 1, position 1, transcriptional unit for transient expression of major latex protein+H34like (NmMLPL) from Nepeta mussinii driven by 35S promoter |
| 177052 | pEPQD1CB0120 | MoClo Level 1, position 1, transcriptional unit for transient expression of major latex protein-like (NmMLPL) from Nepeta mussinii driven by 35S promoter; contains a chloroplast transit peptide |
| 177053 | pEPQD1CB0121 | MoClo Level 1, position 2, transcriptional unit for transient expression of iridoid oxidase; CYP76A26 (CrIO) from Catharanthus roseus driven by 35S promoter |
| 177054 | pEPQD1CB0122 | MoClo Level 1, position 2, transcriptional unit for transient expression of iridoid oxidase; CYP76A26 (CrIO) from Catharanthus roseus driven by 35S promoter; contains a chloroplast transit peptide |
| 177055 | pEPQD1CB0123 | MoClo Level 1, position 3, transcriptional unit for transient expression of 7-deoxyloganetic acid glucosyl transferase (Cr7-DLGT) from Catharanthus roseus driven by 35S promoter |
| 177056 | pEPQD1CB0124 | MoClo Level 1, position 3, transcriptional unit for transient expression of 7-deoxyloganetic acid glucosyl transferase (Cr7-DLGT) from Catharanthus roseus driven by 35S promoter; contains a chloroplast transit peptide |
| 177057 | pEPQD1CB0767 | MoClo Level 1, position 4, transcriptional unit for transient expression of 7-deoxyloganic acid hydroxylase (Cr7-DLH) from Catharanthus roseus driven by 35S promoter |
| 177058 | pEPQD1CB0768 | MoClo Level 1, position 4, transcriptional unit for transient expression of 7-deoxyloganic acid hydroxylase (Cr7-DLH) from Catharanthus roseus driven by 35S promoter; contains a chloroplast transit peptide |
| 177059 | pEPQD1CB0769 | MoClo Level 1, position 5, transcriptional unit for transient expression of loganic acid O-methyltransferase (CrLAMT) from Catharanthus roseus driven by 35S promoter |
| 177060 | pEPQD1CB0770 | MoClo Level 1, position 5, transcriptional unit for transient expression of loganic acid O-methyltransferase (CrLAMT) from Catharanthus roseus driven by 35S promoter; contains a chloroplast transit peptide |
| 177061 | pEPQD1CB0771 | MoClo Level 1, position 6, transcriptional unit for transient expression of secologanin synthase (CrSLS1) from Catharanthus roseus driven by 35S promoter |
| 177062 | pEPQD1CB0772 | MoClo Level 1, position 6, transcriptional unit for transient expression of secologanin synthase (CrSLS1) from Catharanthus roseus driven by 35S promoter; contains a chloroplast transit peptide |
| 177063 | pEPQD1CB0773 | MoClo Level 1, position 1, transcriptional unit for transient expression of tryptophan decarboxylase (CrTDC) from Catharanthus roseus driven by 35S promoter |
| 177064 | pEPQD1CB0774 | MoClo Level 1, position 1, transcriptional unit for transient expression of tryptophan decarboxylase (CrTDC) from Catharanthus roseus driven by 35S promoter; contains a chloroplast transit peptide |
| 177065 | pEPQD1CB0775 | MoClo Level 1, position 2, transcriptional unit for transient expression of strictosidine synthase (CrSTR) from Catharanthus roseus driven by 35S promoter |
| 177066 | pEPQD1CB0776 | MoClo Level 1, position 2, transcriptional unit for transient expression of strictosidine synthase (CrSTR) from Catharanthus roseus driven by 35S promoter; contains a chloroplast transit peptide |
| 177067 | pEPQD1CB0911 | MoClo Level 1, position 5, transcriptional unit for transient expression of loganic acid O-methyltransferase (MsLAMT) from Mitragyna speciosa driven by 35S promoter |
| 177068 | pEPQD1CB0905 | MoClo Level 1, position 1, transcriptional unit for transient expression of gal4AD-phiC31 driven by 35S promoter |
| 177069 | pEPQD1CB0784 | MoClo Level 1, position 1, transcriptional unit for transient expression of P19 suppressor of gene silencing from Tomato Bushy Stunt Virus driven by nos promoter |
| 177070 | pEPQD1CB0785 | MoClo Level 1, position 2, transcriptional unit for transient expression of 1-deoxy-D-xylulose 5-phosphate synthase (CrDXS2) from Catharanthus roseus promoter regions contains 4x attB sites for recruitment fo gal4AD-phiC31 |
| 177071 | pEPQD1CB0786 | MoClo Level 1, position 3, transcriptional unit for transient expression of geranyl pyrophosphate synthase (PaGPPS1) from Picea abies promoter regions contains 4x attB sites for recruitment fo gal4AD-phiC31 |
| 177072 | pEPQD1CB0787 | MoClo Level 1, position 4, transcriptional unit for transient expression of geraniol synthase (CrGES) from Catharanthus roseus promoter regions contains 4x attB sites for recruitment fo gal4AD-phiC31 |
| 177073 | pEPQD1CB0794 | MoClo Level 1, position 3, transcriptional unit for transient expression of geraniol 8-oxidase (CrG8H) from Catharanthus roseus promoter regions contains 4x attB sites for recruitment fo gal4AD-phiC31 |
| 177074 | pEPQD1CB0796 | MoClo Level 1, position 4, transcriptional unit for transient expression of 8-hydroxygeraniol oxidoreductase (CrGOR) from Catharanthus roseus promoter regions contains 4x attB sites for recruitment fo gal4AD-phiC31 |
| 177075 | pEPQD1CB0790 | MoClo Level 1, position 1, transcriptional unit for transient expression of iridoid synthase (CrISY) from Catharanthus roseus promoter regions contains 4x attB sites for recruitment fo gal4AD-phiC31 |
| 177076 | pEPQD1CB0792 | MoClo Level 1, position 2, transcriptional unit for transient expression of major latex protein-like (NmMLPL) from Nepeta mussinii promoter regions contains 4x attB sites for recruitment fo gal4AD-phiC31 |
| 177077 | pEPQD1CB0798 | MoClo Level 1, position 5, transcriptional unit for transient expression of iridoid oxidase; CYP76A26 (CrIO) from Catharanthus roseus promoter regions contains 4x attB sites for recruitment fo gal4AD-phiC31 |
| 177078 | pEPQD1CB0861 | MoClo Level 1, position 6, transcriptional unit for transient expression of 7-deoxyloganetic acid glucosyl transferase (Cr7-DLGT) from Catharanthus roseus promoter regions contains 4x attB sites for recruitment fo gal4AD-phiC31 |
| 177079 | pEPQD1CB0804 | MoClo Level 1, position 2, transcriptional unit for transient expression of 7-deoxyloganic acid hydroxylase (Cr7-DLH) from Catharanthus roseus promoter regions contains 4x attB sites for recruitment fo gal4AD-phiC31 |
| 177080 | pEPQD1CB0806 | MoClo Level 1, position 3, transcriptional unit for transient expression of loganic acid O-methyltransferase (CrLAMT) from Catharanthus roseus promoter regions contains 4x attB sites for recruitment fo gal4AD-phiC31 |
| 177081 | pEPQD1CB0863 | MoClo Level 1, position 5, transcriptional unit for transient expression of secologanin synthase (CrSLS1) from Catharanthus roseus promoter regions contains 4x attB sites for recruitment fo gal4AD-phiC31 |
| 177082 | pEPQD1CB0808 | MoClo Level 1, position 4, transcriptional unit for transient expression of tryptophan decarboxylase (CrTDC) from Catharanthus roseus promoter regions contains 4x attB sites for recruitment fo gal4AD-phiC31 |
| 177083 | pEPQD1CB0802 | MoClo Level 1, position 1, transcriptional unit for transient expression of strictosidine synthase (CrSTR) from Catharanthus roseus promoter regions contains 4x attB sites for recruitment fo gal4AD-phiC31 |
| 177084 | pEPQD1CB0865 | MoClo Level 1, position 6, transcriptional unit for transient expression of geraniol 8-oxidase (CrG8H) from Catharanthus roseus promoter regions contains 4x attB sites for recruitment fo gal4AD-phiC31 |
| 177085 | pEPQD1CB0867 | MoClo Level 1, position 3, transcriptional unit for transient expression of 7-deoxyloganetic acid glucosyl transferase (Cr7-DLGT) from Catharanthus roseus promoter regions contains 4x attB sites for recruitment fo gal4AD-phiC31 |
| 177086 | pEPQD1CB0869 | MoClo Level 1, position 5, transcriptional unit for transient expression of 7-deoxyloganic acid hydroxylase (Cr7-DLH) from Catharanthus roseus promoter regions contains 4x attB sites for recruitment fo gal4AD-phiC31 |
| 177087 | pEPQD1CB0871 | MoClo Level 1, position 2, transcriptional unit for transient expression of secologanin synthase (CrSLS1) from Catharanthus roseus promoter regions contains 4x attB sites for recruitment fo gal4AD-phiC31 |
| 177088 | pEPQD2KN0908 | MoClo Level 2 plasmid containing multiple transcriptional units for transient expression of P19 (cytosol, driven by nosP), along with CrDXS2, PaGPPS1, and CrGES (plastid, driven by 4x opaatB) |
| 177089 | pEPQDPKN0892 | MoClo Level P, position 1, plasmid containing multiple transcriptional units for transient expression of CrISY, NmMLPL, CrG8H, CrGOR, CrIO, and Cr7-DLGT driven by 4x opaatB |
| 177090 | pEPQD2KN0897 | MoClo Level 2 plasmid containing multiple transcriptional units for transient expression of CrISY, NmMLPL, Cr7-DLGT, CrGOR, CrIO, and CrG8H driven by 4x opaatB |
| 177091 | pEPQD2KN0895 | MoClo Level 2 plasmid containing multiple transcriptional units for transient expression of CrSTR, Cr7-DLH, CrLAMT, CrTDC, and CrSLS driven by 4x opaatB |
| 177092 | pEPQD2KN0899 | MoClo Level 2 plasmid containing multiple transcriptional units for transient expression of CrSTR, CrSLS, CrLAMT, CrTDC, and Cr7-DLH driven by 4x opaatB |
| 185623 | pEPQD0CM0032 | Level 0 promoter from N. benthamiana used for sgRNA expression, nonstandard overlaps, GGAG-TTCG |
| 185624 | pEPQD0CM0033 | Level 0 promoter from N. benthamiana used for sgRNA expression, nonstandard overlaps, GGAG-CTCG |
| 185625 | pEPQD1CB0001 | MoClo Level 1, position 2 reverse, transcriptional unit for expression of plant codon optimized Cas9 from Streptococcus pyogenes driven by 35S promoter |
| 185626 | pEPQD1CB0202 | MoClo Level 1, position 5 reverse, transcriptional unit for expression of plant codon optimized Cas9 from Streptococcus pyogenes driven by 35S promoter |
| 185627 | pEPQD0KN0750 | Modified tobacco rattle virus RNA2 acceptor vector that could be used for expression of sgRNAs, contains restriction enzyme sites for golden gate assembly using AarI |
| 185628 | pEPQD0KN0751 | Modified tobacco rattle virus RNA2 acceptor vector that could be used for expression of sgRNAs, contains restriction enzyme sites for golden gate assembly using BsaI |
| 185629 | pEPQDKN0760 | Modified tobacco rattle virus RNA2 vector expressing an sgRNA fused to trucated flowering locus T (BsaI-domesticated) which targets phytoene desaturase (PDS) of Nicotiana benthamiana |
| 185630 | pEPQDKN0761 | Modified tobacco rattle virus RNA2 vector expressing an sgRNA fused to trucated flowering locus T which targets phytoene desaturase (PDS) of Nicotiana benthamiana, functionally equivalent to pEE393 |