PEAR, a flexible fluorescent reporter for the identification and enrichment of successfully prime edited cells.
Simon DA, Talas A, Kulcsar PI, Biczok Z, Krausz SL, Varady G, Welker E
Elife. 2022 Feb 23;11. pii: 69504. doi: 10.7554/eLife.69504. PubMed Article
Simon DA, Talas A, Kulcsar PI, Biczok Z, Krausz SL, Varady G, Welker E
Elife. 2022 Feb 23;11. pii: 69504. doi: 10.7554/eLife.69504. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 80453 | pX330-Flag-wtSpCas9-H840A | SpCas9 mammalian expression vector |
| 177178 | pDAS12125_PEAR-GFP | PEAR-GFP target plasmid with split EGFP and disrupted 5' splice site |
| 177179 | pDAS12124_PEAR-GFP-preedited | PEAR-GFP control plasmid with split EGFP and intact 5' splice site |
| 177180 | pDAS12069_U6-pegRNA-mCherry | pegRNA cloning plasmid with mCherry marker |
| 177181 | pDAS12222_U6-pegRNA-BFP | pegRNA cloning plasmid with BFP marker |
| 177182 | pDAS12230_pegRNA-PEAR-GFP(10PBS-24RT)-mCherry | plasmid expressing a pegRNA targeting the PEAR-GFP plasmid along with an mCherry marker |
| 177183 | pDAS12137_sgRNA-PEAR-GFP-nick(+17)-mCherry | plasmid expressing an sgRNA targeting the PEAR-GFP plasmid along with an mCherry marker |
| 177184 | pDAS12342_PEAR-GFP_2in1 | PEAR-GFP plasmid with a pegRNA that targets its own plasmid |
| 177185 | pDAS12395_PEAR-GFP_2.0-preedited | PEAR-GFP3 control plasmid with split EGFP and intact 5' splice site |
| 177186 | pDAS12489_PEAR-GFP_2in1_2.0 | PEAR-GFP3 plasmid with a pegRNA that targets its own plasmid |