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Robust single-cell discovery of RNA targets of RNA-binding proteins and ribosomes.
Brannan KW, Chaim IA, Marina RJ, Yee BA, Kofman ER, Lorenz DA, Jagannatha P, Dong KD, Madrigal AA, Underwood JG, Yeo GW
Nat Methods. 2021 May;18(5):507-519. doi: 10.1038/s41592-021-01128-0. Epub 2021 May 7. PubMed Article

Plasmids from Article

ID Plasmid Purpose
183901pLIX403_Capture1_APOBEC_HA_P2A_mRubyInducible lentiviral expression, TRE-gateway-APOBEC-HA-P2A-mRuby; PGK-puro-2A-rtTA
183951pLIX403_control_APOBEC_HA_P2A_mRuby_Capture1Inducible lentiviral expression, TRE-control-APOBEC-HA-P2A-mRuby; PGK-puro-2A-rtTA
194703pLIX403_RPS2_APOBEC_HA_P2A_mRuby_Capture1Inducible lentiviral expression, TRE-RPS2-APOBEC-HA-P2A-mRuby; PGK-puro-2A-rtTA (Ribo-STAMP, RPS2)

Antibodies from Article